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Keywords:
Germination characteristics, Mediterranean basin, Metal tolerance, Seedling growth, Soil restoration
Cistus monspeliensis is one of the common plants found on heavy metal (HM) contaminated lands of the Mediterranean region. However, information about the effects of HMs on seed germination and early seedling growth of this shrub is not fully known. This study thus examined the effects of various concentrations (i.e. 0, 200, 400, 600 mg/dm3) of Pb, Cr, Zn, Cd, and Ni on seed germination and early seedling growth of this plant. Seeds germinated maximally in absence of HMs and increases in HM levels led to a decline in the germination. However, seeds could germinate in up to 600 mg/dm3. Generally, Cd was the most and Zn the least inhibitory to germination. Apparent inhibition of seedling growth occurred from the level of 400 mg/dm3 for Ni and Cd. At the higher concentrations (400 and 600 mg/dm3), Pb, Cr and Zn were generally less inhibitory to hypocotyl length than Cd and Ni. This study shows that the tested species is tolerant to increasing concentrations (200–600 mg/dm3) of all HMs, especially Zn, at both seed germination and seedling growth stages. Hence, this species could be considered an excellent candidate for the restoration/rehabilitation of HM-polluted sites under Mediterranean bioclimatic conditions.
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Abdel-Baki AA, Anderson JD (1970) Viability and leaching of sugars from germinating barley. Crop Sci 10(1):31–34. https://doi.org/10.2135/cropsci1970.0011183X001000010012x
Abdu N, Agbenin JO, Buerkert A (2011) Geochemical assessment, distribution, and dynamics of trace elements in urban agricultural soils under long-term wastewater irrigation in Kano, northern Nigeria. J Plant Nutr Soil Sci 174(3):447–458. https://doi.org/10.1002/jpln.201000333
Abraham K, Sridevi R, Suresh B, Damodharam T (2013) Effect of heavy metals (cd, pb, Cu) on seed germination of Arachis hypogeae L. Asian J Plant Sci 3:10–12. https://www.imedpub.com/articles-pdfs/effect-of-heavy-metals-cd-pb-cu-on-seed-germination-ofarachis-hypogeae-l.pdf
Abreu MM, Santos ES, Fernandes E, Magalhães MCF (2012) Trace elements tolerance, accumulation and translocation in Cistus populifolius, Cistus salviifolius and their hybrid growing in polymetallic contaminated mine areas. J Geochem Explor 123:52–60. https://doi.org/10.1016/j.gexplo.2012.05.001
Abreu MM, Magalhães MCF (2009) Phytostabilization of soils in mining areas. Case studies from Portugal. In: Aachen L, Eichmann P (eds) Soil remediation. Nova Science, New York, pp 297–344
Ahmad MSA, Hussain M, Ashraf M, Ahmad R, Ashraf MY (2009) Effect of nickel on seed germinability of some elite sunflower (Helianthus annuus L.) cultivars. Pak J Bot 41(4):1871–1882
Ahmad MSA, Riffat A, Hussain M, Hameed M, Alvi AK (2023) Toxicity and tolerance of nickel in sunflower (Helianthus annuusL). Environ Sci Pollut Res 30:50346–50363. https://doi.org/10.1007/s11356-023-25705-2
Aljasmi M, El-Keblawy A, Mosa KA (2021) Abiotic factors controlling germination of the multipurpose invasive Prosopis pallida: toward aforestation of salt-affected lands in the subtropical arid arabian desert. Trop Ecol 62:116–125. https://doi.org/10.1007/s42965-020-00124-3
Arenas-Lago D, Santos ES, Carvalho LC, Abreu MM, Andrade ML (2018) Cistus monspeliensis L. as a potential species for rehabilitation of soils with multielemental contamination under Mediterranean conditions. Environ Sci Pollut Res 25:6443–6455. https://doi.org/10.1007/s11356-017-0957-3
Barceló J, Poschenrieder C, Andreu I, Gunsé B (1986) Cadmium-induced decrease of water stress resistance in bush bean plants (Phaseolus vulgaris L. Cv. Contender) I. effects of Cd on water potential, relative water content, and cell wall elasticity. J Plant Physiol 125(1–2):17–25. https://doi.org/10.1016/S0176-1617(86)80239-5
Barceló J, Poschenrieder C (1990) Plant water relations as affected by heavy metal stress: a review. J Plant Nutr 13(1):1–37. https://doi.org/10.1080/01904169009364057
Baruah N, Mondal SC, Farooq M, Gogoi N (2019) Influence of heavy metals on seed germination and seedling growth of wheat, pea, and tomato. Water Air Soil Pollut 230:273. https://doi.org/10.1007/s11270-019-4329-0
Batista MJ, Gonzalez-Fernandez O, Abreu MM, Queralt I, Carvalho ML (2017) Pioneer Mediterranean shrub species revegetating soils developed on mining soils/spoils. Land Degrad Dev 28(2):718–730. https://doi.org/10.1002/ldr.2639
Benavides MP, Gallego SM, Tomaro ML (2005) Cadmium toxicity in plants. Braz J Plant Physiol 17:21–34. https://doi.org/10.1590/S1677-04202005000100003
Booker FL (2004) Influence of ozone on ribonuclease activity in wheat (Triticum aestivum) leaves. Physiol Plant 120(2):249–255. https://doi.org/10.1111/j.0031-9317.2004.0238.x
Bravo S, Amorós JA, Pérez-De-Los-Reyes C, García Navarro FJ, Moreno MM, Sánchez-Ormeño M, Higueras P (2017) Influence of the soil pH in the uptake and bioaccumulation of heavy metal (Fe, Zn, Cu, Pb and Mn) and other elements (ca, K, Al, Sr and Ba) in vine leaves, Castilla-La Mancha (Spain). J Geochem Explor 174:79–83. https://doi.org/10.1016/j.gexplo.2015.12.012
Canu M, Mulè P, Spanu E, Fanni S, Marrone A, Carboni G (2022) Hemp cultivation in soils polluted by cd, pb and zn in the Mediterranean area: sites characterization and phytoremediation in real scale settlement. Appl Sci 12(7):3548. https://doi.org/10.3390/app12073548
Ćurguz VG, Raičević V, Veselinović M, Tabacovic-Tošić M, Vilotić D (2012) Influence of heavy metals on seed germination and growth of Picea abies L. Karst. Pol J Environ Stud 21(2):355–361
Czajka KM, Michael P, Nkongolo K (2019) Differential effects of nickel dosages on in vitro and in vivo seed germination and expression of a high affinity nickel-transport family protein (AT2G16800) in trembling aspen (Populus tremuloides). Ecotoxicology 28:92–102. https://doi.org/10.1007/s10646-018-2003-8
Dadach M, Hameed A, El-Keblawy A (2023) Differential effects of chloride salts on seed germination and seedling growth of Cistus monspeliensis: towards revegetation of the Mediterranean salt-contaminated soils. Flora 299:152209. https://doi.org/10.1016/j.flora.2022.152209
Dadach M, Benajaoud A, Mehdadi Z (2021) Salt and drought effect on germination and initial growth of Lavandula stoechas: a potential candidate for rehabilitation of the Mediterranean disturbed coastal lands. Ekologia (Bratislava) 40:301–311. https://doi.org/10.2478/eko-2021-0032
El Rasafi T, Nouri M, Bouda S, Haddioui A (2016) The effect of cd, Zn and Fe on seed germination and early seedling growth of wheat and bean. Ekologia (Bratislava) 35(3):213–223. https://doi.org/10.1515/eko-2016-0017
Fenu G, Carboni M, Acosta ATR, Bacchetta G (2013) Environmental factors influencing coastal vegetation pattern: new insights from the Mediterranean basin. Folia Geobot 48:493–508. https://doi.org/10.1007/s12224-012-9141-1
Fernández-Mazuecos M, Vargas P (2011) Genetically depauperate in the continent but rich in oceanic islands: Cistus monspeliensis (Cistaceae) in the Canary Islands. PLoS ONE 6(2):e17172. https://doi.org/10.1371/journal.pone.0017172
Ferreira CSS, Seifollahi-Aghmiuni S, Destouni G, Ghajarnia N, Kalantari Z (2022) Soil degradation in the European Mediterranean region: processes, status and consequences. Sci Total Environ 5:150106. https://doi.org/10.1016/j.scitotenv.2021.150106
Fryzova R, Pohanka M, Martinkova P, Cihlarova H, Brtnicky M, Hladky J, Kynicky J (2017) Oxidative stress and heavy metals in plants. In: de Voogt P (ed) Reviews of Environmental Contamination and Toxicology, vol 245. Springer, Cham. https://doi.org/10.1007/398_2017_7
Gonzalez-Mendoza D, Zapata-Perez O (2008) Mechanisms of tolerance to potentially toxic elements in plants. Bol Soc Bot México 82:53–61 [In Spanish]. https://doi.org/10.17129/botsci.1781
Gupta V, Jatav PK, Verma R, Kothari SL, Kachhwaha S (2017) Nickel accumulation and its effect on growth, physiological and biochemical parameters in millets and oats. Environ Sci Pollut Res 24:23915–23925. https://doi.org/10.1007/s11356-017-0057-4
Haida S, Bakkouche K, Kribii AR, Kribii A (2021) Chemical composition of essential oil, phenolic compounds content, and antioxidant activity of Cistus monspeliensis from northern Morocco. Biochem Res Int 2021:6669877. https://doi.org/10.1155/2021/6669877
Hassan MU, Chattha MU, Khan I, Chattha MB, Aamer M, Nawaz M, Ali A, Khan MAU, Khan TA (2019) Nickel toxicity in plants: reasons, toxic effects, tolerance mechanisms, and remediation possibilities—a review. Environ Sci Pollut Res 26:12673–12688. https://doi.org/10.1007/s11356-019-04892-x
Kadis CC, Georghiou K (2010) Seed dispersal and germination behavior of three threatened endemic labiates of Cyprus. Plant Species Biol 25(1):77–84. https://doi.org/10.1111/j.1442-1984.2009.00265.x
Karahan F, Ozyigit II, Saracoglu IA, Yalcin IE, Ozyigit AH, Ilcim A (2020) Heavy metal levels and mineral nutrient status in different parts of various medicinal plants collected from eastern Mediterranean region of Turkey. Biol Trace Elem Res 197:316–329. https://doi.org/10.1007/s12011-019-01974-2
Lozano-Isla F, Alfaro OB, Pompelli MF (2019) GerminaR: an R package for germination analysis with the interactive web application ’GerminaQuant for R. Ecol Res 34(2):339–346. https://doi.org/10.1111/1440-1703.1275
Makhniova S, Mohnachev P, Ayan S (2019) Seed germination and seedling growth of scots pine in technogenically polluted soils as container media. Environ Monit Assess. https://doi.org/10.1007/s10661-019-7249-y. 191:113.
Márquez-García B, Márquez C, Sanjosé I, Nieva FJJ, Rodríguez-Rubio P, Muñoz-Rodríguez AF (2013) The effects of heavy metals on germination and seedling characteristics in two halophyte species in Mediterranean marshes. Mar Pollut Bull 70:119–124. https://doi.org/10.1016/j.marpolbul.2013.02.019
Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, London
Martin JAR, Gutierrez C, Torrijos M, Nanos N (2018) Wood and bark of Pinus halepensis as archives of heavy metal pollution in the Mediterranean Region. Environ Pollut 239:438–447. https://doi.org/10.1016/j.envpol.2018.04.036
Masindi V, Muedi KL (2018) Environmental contamination by heavy metals. [In] Heavy Metals (pp. 115–133). IntechOpen. https://doi.org/10.5772/intechopen.76082
Mbadra C, Gargouri K, Ben Mbarek H, Trabelsi L, Arous A, Chaabouni SE (2019) Effect of near-road soil contamination on Solanum lycopersicum L., Cicer arietinum L. and Cucumis sativus L. Int J Environ Sci Technol 16:3467–3482. https://doi.org/10.1007/s13762-018-2033-z
Mendoza-Fernández AJ, Fernández-Ceular Á, Alcaraz-Segura D, Ballesteros M, Peñas J (2022) The fate of endemic species specialized in island habitat under climate change in a Mediterranean high mountain. Plants 11(23):3193. https://doi.org/10.3390/plants11233193
Mishra B, Chandra M (2022) Evaluation of phytoremediation potential of aromatic plants: a systematic review. J Appl Res Med Aromat Plants 31:10040. https://doi.org/10.1016/j.jarmap.2022.100405
Moreira IN, Martins LL, Mourato MP (2020) Effect of cd, cr, Cu, Mn, Ni, Pb and Zn on seed germination and seedling growth of two lettuce cultivars (Lactuca sativa L). Plant Physiol Rep 25(2):347–358. https://doi.org/10.1007/s40502-020-00509-5
Munzuroglu O, Geckil H (2002) Effects of metals on seed germination, root elongation, and coleoptile and hypocotyl growth in Triticum aestivum and Cucumis sativus. Arch Environ Contam Toxicol 43:203–213. https://doi.org/10.1007/s00244-002-1116-4
Nedjimi B, Daoud Y (2009) Ameliorative effect of CaCl2 on growth, membrane permeability and nutrient uptake in Atriplex halimus subsp. schweinfurthii grown at high (NaCl) salinity. Desalination 249:163–166. https://doi.org/10.1016/j.desal.2009.01.019
Nedjimi B (2021) Phytoremediation: a sustainable environmental technology for heavy metals decontamination. SN Appl Sci 3:286. https://doi.org/10.1007/s42452-021-04301-4
Nedjimi B (2020) Germination characteristics of Peganum harmala L. (Nitrariaceae) subjected to heavy metals: implications for the use in polluted dryland restoration. Int J Environ Sci Technol 17:2113–2122. https://doi.org/10.1007/s13762-019-02600-3
Nunes JR, Ramos-Miras J, Lopez-Piñeiro A, Loures L, Gil C, Coelho J, Loures A (2014) Concentrations of available heavy metals in Mediterranean agricultural soils and their relation with some soil selected properties: a case study in typical Mediterranean soils. Sustainability 6(12):9124–9138. https://doi.org/10.3390/su6129124
Osman HE, Fadhlallah RS (2023) Impact of lead on seed germination, seedling growth, chemical composition, and forage quality of different varieties of Sorghum. J Umm Al-Qura Univ Appll Sci 9:77–86. https://doi.org/10.1007/s43994-022-00022-5
Ozdener Y, Kutbay HG (2009) Toxicity of copper, cadmium, nickel, lead and zinc on seed germination and seedling growth in Eruca sativa. Fresenius Environ Bull 18:26–31
Ranal MA, Santana DG (2006) How and why to measure the germination process? Rev Bras Bot 29(1):1–11. https://doi.org/10.1590/S0100-84042006000100002
Rizwan M, Ali S, Qayyum MF, Ok YS, Zia-ur-Rehman M, Abbas Z, Hanna F (2017) Use of Maize (Zea mays L.) for phytomanagement of Cd-contaminated soils: a critical review. Environ Geochem Health 39:259–277. https://doi.org/10.1007/s10653-016-9826-0
Rodríguez-Torres P (2013) Evaluación fisiológica de species vegetales que crecen en sitios impactados por residues generadores de drenaje ácido de roca [Physiological evaluation of plant species that grow in sites impacted by residues generating acid rock drainage] [dissertation]. San Luis Potosí (México): Universidad Autónoma de San Luis Potosí. [In Spanish]
Rout GR, Samantaray S, Das P (2000) Effects of chromium and nickel on germination and growth in tolerant and non-tolerant populations of Echinochloa colona (L.) Link. Chemosphere 40(8):855–859. https://doi.org/10.1016/s0045-6535(99)00303-3
Santos ES, Abreu MM, Magalhães MCF (2016) Cistus ladanifer phytostabilizing soils contaminated with non-essential chemical elements. Ecol Eng 94:107–116. https://doi.org/10.1016/j.ecoleng.2016.05.072
Sayah K, Chemlal L, Marmouzi I, El Jemli M, Cherrah Y, Faouzi MA (2017) In vivo anti-inflammatory and analgesic activities of Cistus salviifolius (L.) and Cistus monspeliensis (L.) aqueous extracts. S Afr J Bot 113:160–163. https://doi.org/10.1016/j.sajb.2017.08.015
Sethy SK, Ghosh S (2013) Effect of heavy metals on germination of seeds. J Nat Sci Biol Med 4(2):272–275. https://doi.org/10.4103/0976-9668.116964
Shafiq M, Iqbal MZ (2006) The toxicity effects of heavy metals on germination and seedling growth of Cassia siamea Lamk. J New Seeds 7(4):95–105. https://doi.org/10.1300/J153v07n04_07
Shahid M, Dumat C, Khalid S, Niazi NK, Antunes PMC (2016) Cadmium bioavailability, uptake, toxicity and detoxification in soil-plant system. In: de Voogt P (ed) Reviews of environmental contamination and toxicology volume 241. Springer, Cham. https://doi.org/10.1007/398_2016_8
Shokr MS, Abdellatif MA, El Behairy RA, Abdelhameed HH, El Baroudy AA, Mohamed ES, Rebouh NY, Ding Z, Abuzaid AS (2022) Assessment of potential heavy metal contamination hazards based on GIS and multivariate analysis in some Mediterranean zones. Agronomy 12(12):3220. https://doi.org/10.3390/agronomy12123220
Siddiqui MH, AlWhaibi MH, Basalah MO (2011) Interactive effect of calcium and gibberellin on nickel tolerance in relation to antioxidant systems in Triticum aestivum L. Protoplasma 248:503–5110. https://doi.org/10.1007/s00709-010-0197-6
Sirkou D, Alados CL, Papanastasis VP, Vrahnakis MS, Iovi K, Giner ML, Ispikoudis I (2002) Assessment of grazing effects on Mediterranean shrubs Phillyrea Latifolia L. and Cistus monspeliensis L. with developmental instability and fractal dimension. J Mediterr Ecol 3(4):19–29
Tesema AA, Mekonen DA (2023) Responses of Niger [Guizotia abyssinica (L.f.) Cass.] To cadmium and nickel stress. Vegetos. https://doi.org/10.1007/s42535-023-00591-8
Thakur S, Sharma SS (2016) Characterization of seed germination, seedling growth, and associated metabolic responses of Brassica juncea L. cultivars to elevated nickel concentrations. Protoplasma 253:571–580. https://doi.org/10.1007/s00709-015-0835-0
Tiwari S, Lata C (2018) Heavy metal stress, signaling, and tolerance due to plant-associated microbes: an overview. Front Plant Sci 9:452. https://doi.org/10.3389/fpls.2018.00452
Trabaud L, Oustric J (1989) Heat requirements for seed germination of three cistus species in the garrigue of southern France. Flora 183(3–4):321–325. https://doi.org/10.1016/S0367-2530(17)31557-8
Van Bussel CGJ, Schroeder JP, Mahlmann L, Schulz C (2014) Aquatic accumulation of dietary metals (Fe, Zn, Cu, Co, Mn) in recirculating aquaculture systems (RAS) changes body composition but not performance and health of juvenile turbot (Psetta maxima). Aquac Eng 61:35–42. https://doi.org/10.1016/j.aquaeng.2014.05.003
Yáñez-Espinosa L, Briones-Gallardo R, Flores J, del Castillo EÁ (2020) Effect of heavy metals on seed germination and seedling development of Nama aff. Stenophylla collected on the slope of a mine tailing dump. Int J Phytorem 22(14):1448–1461. https://doi.org/10.1080/15226514.2020.1781782
The authors would like the thank la Direction Générale de la Recherche Scientifique et du Développement Technologique (D.G.R.S.D.T., le Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, Algérie) for supporting this work. We are grateful to all persons who contributed in the lab investigations.