*Article not assigned to an issue yet
Ali Fayaz, Kainat, Khan Waqar, Khan Alamgir, Jan Amin Ullah, Amin Muhammad, Musarella Carmelo Maria
Keywords:
Chlorophyll, Growth indices, Indole acetic acid, Anthocyanin, n Brassicaceae Rapa L., Vitamin B6
Role of vitamin B6 (pyridoxal HCL) is well known in different enzymatic reactions, cellular processes and in oxidative stress responses. Pathway for the natural synthesis of pyridoxal has been identified by several workers. A total of 24 genes, actively involved in the synthesis of vitamin B6 were identified from Arabidopsis thaliana (14 genes) and Brassica rapa (10 genes). Field experiment was conducted on B. rapa varieties (white and purple cap) to study the effect of three different levels of pyridoxine (25, 50 and 75 ppm) for 15 days during March. Growth indices were recorded on 15th day after sowing for control, test-I (25 ppm), test-II (50ppm) and test-III (75ppm). The germination time of seeds was reduced by two days for test-III in comparison to control. Similarly, growth indices like total fresh material (TFM), total dry material (TDM), root and shoot length, chlorophyll contents, carotenoids, indole acetic acid and anthocyanin pigments were also calculated for both B. rapa varieties. Apart from anthocyanin, all the recorded parameters shows significant increase for test-III (75 ppm) followed by test-I (25 ppm), test-II (50ppm) and control group respectively. Similarly, chlorophyll b contents for purple cap Brassica shows an uneven pattern, i.e. initially decreased with pyridoxine of 25 ppm in comparison to control and then increased with 50 ppm and 75 ppm treatments. The results of the current study expose the positive effect of pyridoxine in enhancing growth indices of B. rapa which will in turn leads to increase in yield of this important cash crop.
(*Only SPR Life Members can get full access.)
Abenavoli L, Milanovic M, Procopio AC, Spampinato G, Maruca G, Perrino EV, Mannino GC, Fagoonee S, Luzza F, Musarella CM (2021) Ancient wheats: beneficial effects on insulin resistance. Minerva Med 12(5):641–650. https://doi.org/10.23736/S0026-4806.20.06873-1
Ali F, Khan N (2022) Do environmental variables and overstory communities affect the spatial pattern of understory vegetation? Lessons from Monotheca buxifolia (Falc.) A. DC. forests in Pakistan. Acta Botanica Brasilica, 36. https://doi.org/10.1590/0102-33062021abb0210
Ali F, Khan N, Rahmonov O (2022) Ecosystem Services and Linkages of Naturally Managed Monotheca buxifolia (Falc.) A. DC. Forests with Local Communities across Contiguous Mountainous Ranges in Pakistan. Biology, 11(10), 1469. https://doi.org/10.3390/biology11101469
Ali F, Khan N, Ali K, Amin M, Khan MEH, Jones DA (2023) Assessment of variability in nutritional quality of wild edible fruit of Monotheca buxifolia (Falc.) A. DC. Along the altitudinal gradient in Pakistan. Saudi Journal of Biological Sciences, 30(1), 103489. https://doi.org/10.1016/j.sjbs.2022.103489
Amin M, Gurmani AR, Ali F, Khan SM, Farid A, Shakur M, Khan W (2022) Investigation of multi-pesticide residues in Prunus persica L.(peach) cultivars of district swat using gas chromatography-mass spectroscopy. Pol J Environ Stud 31:1535–1542. https://doi.org/10.1016/j.sjbs.2021.06.035
Arias T, Beilstein MA, Tang M, McKain MR, Pires JC (2014) Diversification times among Brassica (Brassicaceae) crops suggest hybrid formation after 20 million years of divergence. Am J Bot 101(1):86–91. https://doi.org/10.3732/ajb.1300312
Bhat MN, Singh B, Surmal O, Singh B, Shivgotra V, Musarella CM (2021) Ethnobotany of the himalayas: Safeguarding Medical practices and traditional uses of Kashmir regions. Biology 10:851. https://doi.org/10.3390/biology10090851
Bowen-Forbes CS, Zhang Y, Nair MG (2010) Anthocyanin content, antioxidant, anti-inflammatory and anticancer properties of blackberry and raspberry fruits. J Food Compos Anal 23(6):554–560. https://doi.org/10.1016/j.jfca.2009.08.012
Calderón-Ospina CA, Nava‐Mesa MO (2020) B vitamins in the nervous system: current knowledge of the biochemical modes of action and synergies of thiamine, pyridoxine, and cobalamin. CNS Neurosci Ther 26(1):5–13. https://doi.org/10.1111/cns.13207
Cassán F, Vanderleyden J, Spaepen S (2014) Physiological and agronomical aspects of phytohormone production by model plant-growth-promoting rhizobacteria (PGPR) belonging to the genus Azospirillum. J Plant Growth Regul 33(2):440–459. https://doi.org/10.1007/s00344-013-9362-4
Chen R, Shimono A, Aonso M, Nakajima N, Ohsawa R, Yoshioka Y (2020) Genetic diversity and population structure of feral rapeseed (Brassica napus L.) in Japan. PLoS ONE 15(1):e0227990. https://doi.org/10.1371/journal.pone.0227990
Chen S, Wan Z, Nelson MN, Chauhan JS, Redden R, Burton WA, Lin P, Salisbury PA, Fu T, Cowling WA (2013) Evidence from genome-wide simple sequence repeat markers for a polyphyletic origin and secondary centers of genetic diversity of Brassica juncea in China and India. J Hered 104(3):416–427. https://doi.org/10.1093/jhered/est015
Compant S, Mitter B, Colli-Mull JG, Gangl H, Sessitsch A (2011) Endophytes of grapevine flowers, berries, and seeds: identification of cultivable bacteria, comparison with other plant parts, and visualization of niches of colonization. Microb Ecol 62(1):188–197. https://doi.org/10.1007/s00248-011-9883-y
Da Silva VR, Gregory JF III (2020) Vitamin B6. In Present knowledge in nutrition pp. 225–237. Academic Press. https://doi.org/10.1016/B978-0-323-66162-1.00013-5
Dawood MG, El-Sayed El-Awadi M, Abdel-Baky YR, Sadak MH (2017) Physiological role of Ascobin on Quality and Productivity of sunflower plants irrigated with Sodium Chloride Solution. Agricultural Eng International: CIGR J, 16–26
Dejanovic GM, Asllanaj E, Gamba M, Raguindin PF, Itodo OA, Minder B, Kern H (2021) Phytochemical characterization of turnip greens (Brassica rapa ssp. rapa): a systematic review. PLoS ONE 16(2):e0247032. https://doi.org/10.1371/journal.pone.0247032
El Karamany MF, Sadak MS, Bakhoum GS, Omar HAA, Bakry BA (2022) Pyridoxine improving effect on yield, chemical and nutritional value of Egyptian clover plant. Asian J Plant Sci 21(3):360–373. https://doi.org/10.3923/ajps.2022.360.373
El-Bassiouny HMS, Sadak MS (2015) Impact of foliar application of ascorbic acid and α- tocopherol on antioxidant activity and some biochemical aspects of flax cultivars under salinity stress. Acta Biol Colomb 20(2):209–221. https://doi.org/10.15446/abc.v20n2.43868
Gentile C, Spampinato G, Patti M, Laface VLA, Musarella CM (2022) Contribution to the ethnobotanical knowledge of Serre Calabre (Southern Italy). Res J Ecol Environ Sci 2(3):35–55. https://doi.org/10.31586/rjees.2022.389
Hadi H (2016) Simultaneous Spectrophotometric determination of vitamin B6 by coupling with p-Amino phenol. Int J Pharm Clin Res 7(04):84–89
Ioannides C, Konsue N (2015) A principal mechanism for the cancer chemopreventive activity of phenethyl isothiocyanate is modulation of carcinogen metabolism. Drug Metab Rev 47(3):356–373. https://doi.org/10.3109/03602532.2015.1058819
Iqbal MS, Akbar M (2021) Phenotypic diversity analysis and performance of elite lines of Brassica napus L. Bangladesh J Bot 50(1):1–6. https://doi.org/10.3329/bjb.v50i1.52661
Khan W, El-Shehawi AM, Ali F, Ali M, Alqurashi M, Althaqafi MM, Alharthi SB (2023) A genome-wide identification and expression pattern of LMCO Gene Family from Turnip (Brassica rapa L.) under various Abiotic stresses. Plants 12(9):1904. https://doi.org/10.3390/plants12091904
Krinsky NI, Landrum JT, Bone RA (2003) Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye. Annu Rev Nutr 23(1):171–201. https://doi.org/10.1146/annurev.nutr.23.011702.073307
Letunic I, Bork P (2018) 20 years of the SMART protein domain annotation resource. Nucleic Acids Res 46(D1). https://doi.org/10.1093/nar/gkx922. D493-D496
Mangel N, Fudge JB, Li KT, Wu TY, Tohge T, Fernie AR, Vanderschuren H (2019) Enhancement of vitamin B6 levels in rice expressing Arabidopsis vitamin B6 biosynthesis de novo genes. Plant J 99(6):1047–1065. https://doi.org/10.1111/tpj.14379
Perrino EV (2022) Ancient and modern grains: effects on human health: a first short review. Res J Ecol Environ Sci 2(1):21–25. https://doi.org/10.31586/rjees.2022.225
Rady MM, Sadak MS, El-Bassiouny HMS, El-Monem AAA (2011) Alleviation the adverse effects of salinity stress in sunflower cultivars using nicotinamide and α-tocopherol. Aust J Basic Appl Sci 5(10):342–355
Raza A, Hafeez MB, Zahra N, Shaukat K, Umbreen S, Tabassum J, Charagh S, Khan RSA, Hasanuzzaman M (2020) The plant family Brassicaceae: introduction, biology, and importance. The plant family Brassicaceae. Springer, Singapore, pp 1–43. https://doi.org/10.1007/978-981-15-6345-4_1
Sadak MS (2023) Physiological Role of Arbuscular Mycorrhizae and Vitamin B1 on Productivity and Physio-Biochemical Traits of White Lupine (Lupinus termis L.) Under Salt Stress. Gesunde Pflanzen (2023). https://doi.org/10.1007/s10343-023-00855-y
Sadak MS (2022) Biochemical responses of white termis to pyridoxine and mycorrhizae treatment under salinity stress. Egypt J Chem 65(10):429–439. https://doi.org/10.21608/EJCHEM.2022.118032.5319
Sadiq M, Akram NA, Ashraf M (2018) Impact of exogenously applied tocopherol on some key physio-biochemical and yield attributes in mungbean [Vigna radiata (L.) Wilczek] under limited irrigation regimes. Acta Physiol Plant 40(7):1–14. https://doi.org/10.1007/s11738-018-2711-y
Samsatly J, Bayen S, Jabaji SH (2020) Vitamin B6 is under a tight balance during Disease development by Rhizoctonia solani on different cultivars of potato and on Arabidopsis thaliana mutants. Front Plant Sci 11:875. https://doi.org/10.3389/fpls.2020.00875
Schall P, Marutschke L, Grimm B (2020) The flavoproteome of the model plant Arabidopsis thaliana. Int J Mol Sci 21(15):5371. https://doi.org/10.3390/ijms21155371
Senadheera SD, Turchini GM, Thanuthong T, Francis DS (2012) Effects of dietary vitamin B6 supplementation on fillet fatty acid composition and fatty acid metabolism of rainbow trout fed vegetable oil based diets. J Agric Food Chem 60(9):2343–2353. https://doi.org/10.1021/jf204963w
Vollmer J, Johnson BL, Deckard EL, Rahman M (2020) Evaluation of simulated hail damage on seed yield and agronomic traits in canola (Brassica napus L). Can J Plant Sci 100(6):597–608. https://doi.org/10.1139/cjps-2020-0036
Vrolijk MF, Opperhuizen A, Jansen EH, Hageman GJ, Bast A, Haenen GR (2017) The vitamin B6 paradox: supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function. Toxicol in Vitro 44:206–212. https://doi.org/10.1016/j.tiv.2017.07.009
Wang C, Bian C, Xing H, Zhang X, Shen Y (2019) Effect of measure units on estimating crop LEAF chlorophyll content with remote sensing. J Eng 23:8627–8631. https://doi.org/10.1049/joe.2018.9070