Development of micropropagation protocol for Colebrookea oppositifolia Sm. using nodal segments

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00697-z
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Keywords: n Colebrookean , Micropropagation, Shoot induction, LCMS, DNA barcoding, Genetic fidelity


Abstract


In the present study, an attempt was made to optimize the micropropagation protocol for Colebrookea oppositifolia Sm. via direct organogenesis using axillary buds. We also investigated the effect of different concentrations of gibberellic acid (GA3) on seed germination. A very low seed germination percentage was observed in this plant. Murashige and Skoog (MS) medium with 1 mg/L thidiazuron (TDZ) recorded the highest shoot induction percentage of 91.66±1.66a. Further, shoot multiplication was seen to be highest with 7.85±0.057a number of shoots per explant on MS + 2 mg/L 6-benzylaminopurine (BAP) + 0.1 mg/L thidiazuron (TDZ) medium. For rooting, MS medium supplemented with various auxins was used, and the best rooting response with 12.97±0.093a average numbers of roots per shoot and mean root length of 1.205±0.015a cm was seen in half MS medium with 1 mg/L indole-3-butyric acid (IBA). The in vitro raised plantlets were transferred to pots containing the mixture of soil:sand:vermiculite in the ratio of 2:1:1 for hardening. Established plants were shifted to green house and then under field conditions with 90% survival rate. Liquid chromatography mass spectroscopy (LCMS) analysis of tissue culture raised plants marked the presence of therapeutically important plant metabolite, acetoside. DNA barcoding was done to authenticate the explants and tissue culture raised plantlets and sequences were submitted to the GenBank (MZ343371 and MZ343373, respectively). Further, the genetic stability of micropropagated plants were also determined using random amplified polymorphic DNA (RAPD) primers.


n              Colebrookean            , Micropropagation, Shoot induction, LCMS, DNA barcoding, Genetic fidelity


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References


Acharya KP, Acharya M (2010) Traditional knowledge on medicinal plants used for the treatment of livestock diseases in Sardikhola VDC, Kaski, Nepal. J Med Plants Res 4(2):235–239


Ahmad FI, Wagiran A, Abd Samad A, Rahmat Z, Sarmidi MR (2016) Improvement of efficient in vitro regeneration potential of mature callus induced from Malaysian upland rice seed (Oryza sativa cv. Panderas). Saudi J Biol Sci 23(1):S69–S77. https://doi.org/10.1016/j.sjbs.2015.10.022


Ali I, Sharma P, Suri KA, Satti NK, Dutt P, Afrin F, Khan IA (2011) In vitro antifungal activities of amphotericin B in combination with acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia. J Med Microbiol 60(9):1326–1336. https://doi.org/10.1099/jmm.0.031906-0


Banerjee S, Tripathi J, Verma PC, Dwivedi PD, Khanuja SP, Bagchi GD (2004) Thidiazuron-induced high-frequency shoot proliferation in Cineraria maritima Linn. Curr Sci 87(9):1287–1290


Darrudi R, Hassandokht MR, Nazeri V (2015) Effects of moist stratification, GA3 and seed age on seed germination of Rheum khorasanicum B. Baradaran & A. Jafari. J Appl Res Med Aromat Plants 2(4):168–173. https://doi.org/10.1016/j.jarmap.2015.07.001


Dissanayake P, George DL, Gupta ML (2010) Effect of light, gibberellic acid and abscisic acid on germination of guayule (Parthenium argentatum Gray) seed. Ind Crops Prod 32(2):111–7. https://doi.org/10.1016/j.indcrop.2010.03.012


Driver J, Suttle G (1987) Nursery handling of propagules. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry. Springer, Dordrecht, pp 320–335


Fallah M, Farzaneh M, Yousefzadi M, Ghorbanpour M, Mirjalili MH (2019) In vitro mass propagation and conservation of a rare medicinal plant, Zhumeria Majdae Rech. f & Wendelbo (Lamiaceae). Biocatal Agric Biotechnol 17:318–325. https://doi.org/10.1016/j.bcab.2018.12.010


Gaur R (1999) Flora of the District Garhwal, North West Himalaya. Transmedia, USA


Ghaderi S, Ebrahimi SN, Ahadi H, Moghadam SE, Mirjalili MH (2019) In vitro propagation and phytochemical assessment of Perovskia abrotanoides Karel (Lamiaceae)—a medicinally important source of phenolic compounds. Biocatal Agric Biotechnol 19:101113. https://doi.org/10.1016/j.bcab.2019.101113


Ghaisas M, Sharma S, Ganu G, Limaye R (2010) Antiulcer activity of Colebrookea oppositifolia Sm. Res J Pharmacol Pharmacodyn 2(1):66–70


Ghareb HES, Ibrahim SD, Hegazi GAEM (2020) In vitro propagation and DNA barcode analysis of the endangered Silene schimperiana in Saint Katherine protectorate. J Genet Eng Biotechnol 18(1):1–15. https://doi.org/10.1186/s43141-020-00052-8


Goyal AK, Pradhan S, Basistha BC, Sen A (2015) Micropropagation and assessment of genetic fidelity of Dendrocalamus strictus (Roxb.) nees using RAPD and ISSR markers. 3 Biotech 5(4):473–482. https://doi.org/10.1007/s13205-014-0244-7


Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Organ Cult 33(2):105–119. https://doi.org/10.1007/BF01983223


Ishtiaq S, Meo MB, Afridi MSK, Akbar S, Rasool S (2016) Pharmacognostic studies of aerial parts of Colebrookea oppositifolia Sm. Ann Phytomed 5(2):161–167


Jan M, Singh S, Maqbool F (2016) Micropropagation of some medicinally important plant species of family Lamiaceae—a review. Int J Biosci Technol 9(11):64–73


Jogam P, Sandhya D, Shekhawat MS, Alok A, Manokari M, Abbagani S, Allini VR (2020) Genetic stability analysis using DNA barcoding and molecular markers and foliar micro-morphological analysis of in vitro regenerated and in vivo grown plants of Artemisia vulgaris L. Ind Crops Prod 151:112476. https://doi.org/10.1016/j.indcrop.2020.112476


Kaushik P, Dhiman AK (1999) Medicinal plants and raw drugs of India. Bishen Singh Mahendra Pal Singh, India


Kołodziejek J, Patykowski J, Wala M (2017) Effect of light, gibberellic acid and nitrogen source on germination ofeight taxa from dissapearing European temperate forest, Potentillo albae-Quercetum. Sci Rep 7:13924. https://doi.org/10.1038/s41598-017-13101-z


Liu CZ, Murch SJ, EL-Demerdash M, Saxena PK (2003) Regeneration of the Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep 21(6):525–530. https://doi.org/10.1007/s00299-002-0561-x


Madhavan V, Yadav D, Murali A, Yogandrasimha S (2009) Wound healing activity of aqueous and alcohol extracts of leaves of Colebrookea oppositifolia Smith. Indian Drugs 46(3):209–213


Madhavan V, Yadav DK, Gurudeva M, Yoganarasimhan S (2011) Pharmacognostical studies on the leaves of Colebrookea oppositifolia Smith. Asian J Tradit Med 6(4):134–144


Manandhar NP (1995) An inventory of some herbal drugs of Myagdi District, Nepal. Econ Bot 49(4):371–379


Medeiros MJ, Oliveira MT, Willadino L, Santos MG (2015) Overcoming seed dormancy using gibberellic acid and the performance of young Syagrus coronata plants under severe drought stress and recovery. Plant Physiol Biochem 97:278–86. https://doi.org/10.1016/j.plaphy.2015.10.008


Misic D, Grubisic D, Konjevic R (2006) Micropropagation of Salvia brachyodon through nodal explants. Biol Plant 50(3):473–476. https://doi.org/10.1007/s10535-006-0074-5


Mok MC, Mok DW, Turner JE, Mujer CV (1987) Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. Hortscience 22(6):1194–1197. https://doi.org/10.21273/HORTSCI.22.6.1194


Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physiol 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x


Nadkarni K, Nadkarni A (1976) Indian materia medica, vol II. Popular Prakashan Private Limited, Bombay, India


Pavendan P, Rajasekaran CS (2011) Effect of different concentrations of plant growth regulators for micropropagation of Eugenia singampattiana Beddome endangered tree species. Res J Bot 6(3):122


Peron G, Hošek J, Prasad Phuyal G, Raj Kandel D, Adhikari R, Dall’Acqua S (2020) Comprehensive characterization of secondary metabolites from Colebrookea oppositifolia (Smith) leaves from Nepal and assessment of cytotoxic effect and anti-Nf-κB and AP-1 activities in vitro. Int J Mol Sci 21(4):4897. https://doi.org/10.3390/ijms21144897


Sherif NA, Kumar TS, Rao M (2020) DNA barcoding and genetic fidelity assessment of micropropagated Aenhenrya rotundifolia (Blatt.) CS Kumar and FN Rasm.: a critically endangered jewel orchid. Physiol Mol Biol Plants 26:2391–2405. https://doi.org/10.1007/s12298-020-00917-9


Siddique I, Anis M (2007) Rapid micropropagation of Ocimum basilicum using shoot tip explants pre-cultured in thidiazuron supplemented liquid medium. Biol Plant 51(4):787–790. https://doi.org/10.1007/s10535-007-0161-2


Singh M, Saharan V, Rajpurohit D, Sen Y, Joshi A, Sharma A (2017) Thidiazuron induced direct shoot organogenesis in Stevia rebaudiana and assessment of clonal fidelity of regenerated plants by RAPD and ISSR. Int J Curr Microbiol Appl Sci 6(8):1690–1702. https://doi.org/10.20546/ijcmas.2017.608.203


Soumen S, Abdul K, Chandan S, Parthadeb G (2012) In vitro propagation of Ocimum gratissimum L. (Lamiaceae) and its evaluation of genetic fidelity using RAPD marker. Am J Plant Sci 3(1):16627. https://doi.org/10.4236/ajps.2012.31006


Sultan U, Mukhtar A, Ahmad H, Haider A, Ullah H (2016) Conservation status of threatened endemic flora of Western Himalayas. Biodivers Conserv 9(3):91–9


Tejavathi D, Bauvana B (1996) Micropropagation of Solanum viarum Dunal through cotyledonary node, shoot tip and nodal cultures. J Phytol Res 9(2):101–105


Thakur S, Sidhu M (2014) Phytochemical screening of some traditional medicinal plants. Res J Pharm Biol Chem Sci 5(4):1088–1097


Udayan P, Harinarayanan M, Tushar K, Balachandran I (2008) Some common plants used by Kurichiar tribes of Tirunelli forest, Wayanad district, Kerala in medicine and other traditional uses. Indian J Tradit Knowl 7(2):250–255


Viswanath C, Singh’Ratan R (2004) Geographical indications of plant species in ITKs in agriculture. Indian Council of Agricultural Research, New Delhi


Viswanatha GL, Venkataranganna MV, Prasad NBL (2017) Ameliorative potential of Colebrookea oppositifolia methanolic root extract against experimental models of epilepsy: Possible role of GABA mediated mechanism. Biomed Pharmacother 90:455–465. https://doi.org/10.1016/j.biopha.2017.03.078


Viswanatha GL, Venkataranganna MV, Prasad NBL, Hanumanthappa S (2018) Chemical characterization and cerebroprotective effect of methanolic root extract of Colebrookea oppositifolia in rats. J Ethnopharmacol 223:63–75. https://doi.org/10.1016/j.jep.2018.05.009


Viswanatha GL, Shylaja H, Kumar HY, Venkataranganna M, Prasad N (2020) Traditional uses, phytochemistry, and ethnopharmacology of Colebrookea oppositifolia Smith: a mini-review. Adv Tradit Med 21:209–229. https://doi.org/10.1007/s13596-020-00513-y


Yadav DK (2019) Pharmacognostical, Phytochemical and Pharmacological profile of Colebrookea oppositifolia Sm. J Drug Deliv Ther 9:233–237. https://doi.org/10.22270/jddt.v9i6-s.3745


Yadav K, Aggarwal A, Singh N (2013) Evaluation of genetic fidelity among micropropagated plants of Gloriosa superba L. using DNA-based markers—a potential medicinal plant. Fitoterapia 89:265–270. https://doi.org/10.1016/j.fitote.2013.06.009


Yang F, Li XC, Wang HQ, Yang C-R (1996) Flavonoid glycosides from Colebrookea oppositifolia. Phytochemistry 42(3):867–869. https://doi.org/10.1016/0031-9422(95)00975-2


Chopra R, Nayar S, Chopra I (1956) Glossary of Indian medicinal plants. Council of Scientific and Industrial Research, New Delhi


Kumar N, Reddy MP (2012) Thidiazuron (TDZ) induced plant regeneration from cotyledonary petiole explants of elite genotypes of Jatropha curcas: a candidate biodiesel plant. Ind Crops Prod 39:62–8. https://doi.org/10.1016/j.indcrop.2012.02.011


Mehalaine S, Menasria T, Bouguessa S, Yahia A (2017) In vitro seed germination of some Algerian medicinal plants and the effect of gibberellic acid (GA3) on breaking dormancy. J Mater Environ Sci 8(6):2034–2039

 


Acknowledgements


RC is thankful to CSIR (Council of Scientific and Industrial Research) for Senior Research Fellowship. SGG acknowledges CSIR-IIIM (Indian Institute of Integrative Medicine) for support in carrying out the experiments and compilation of the manuscript.


Author Information


Sen Yadunandan
Plant Sciences and Agrotechnology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India

Chouhan Rekha
CSIR-Indian Institute of Integrative Medicine, Jammu, India