Vegetos
(An International Journal of Plant Research & Biotechnology)
Detection of facultative apomixis in little millet, Panicum sumatrense
*Article not assigned to an issue yet
Rathi Sujata, Rani Renu, Srivastava Anand Kumar, Kumar Deepak
Research Articles | Published: 26 September, 2024
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Keywords: Apomixis, n Panicum sumatrensen , Aposporous initial cells, Meiosis, Syngamy
Abstract
In the present investigation, tetraploid small millet P. sumatrense was explored for detailed morphological and embryological analyses of female reproductive structures to find out apomixis. Twenty six accessions of little millet were taken for this study. All the accessions showed sexual embryo sacs of Polygonum type during the course of sexual reproduction. But out of these twenty six accessions, twelve accessions also showed aposporous embryo sacs of Panicum type besides the formation of sexual embryo sac, indicating the occurrence of facultative apomixis. Development of a 4 nucleated Panicum type embryo sac started with the formation of a large vacuole in an aposporous initial having a nucleus at one pole. After two successive mitotic divisions, 4 nuclei organized into one egg cell with two synergids and one polar nucleus to form a Panicum-type embryo sac. These observations indicated the presence of facultative apomixis within P. sumatrense, as both sexual and apomictic reproductive pathways are functional. This fascinating phenomenon of seed production utilizes the organ involved in sexual seed production, however, surpasses essential steps such as meiosis and syngamy. The detection of apomicts in natural populations provides a broad field of research to learn how apomictic crops cope with biotic and abiotic stresses. The findings of the present investigation can be utilized in complex breeding strategies to generate high-yielding nutritionally enriched resistant varieties.
References
Aliyu OM, Schranz ME, Sharbel TF (2010) Quantitative variation for apomictic reproduction in the genus Boechera (Brassicaceae). Am J Bot 97:1719–1731
Barcaccia G, Albertini E (2013) Apomixis in plant reproduction: a novel perspective on an old dilemma. Plant Reprod 26:159–179
Barcaccia G, Varotto S, Meneghetti S, Albertini E, Porceddu A, Parrini P, Lucchin M (2001) Analysis of gene expression during flowering in apomeiotic mutants of Medicago spp: cloning of ESTs and candidate genes for 2n eggs. Sex Plant Reprod 14:233–238
Barke H, Daubert M, Hörandl E (2018) Establishment of apomixis in diploid F2 hybrids and inheritance of apospory from F1 to F2 hybrids of the Ranunculus auricomus complex. Front Plant Sci 9:1111
Beck JB et al (2012) Does hybridization drive the transition to asexuality in diploid Boechera. Evolution 66:985–995
Bicknell RA, Borst NK, Koltunow AM (2000) Monogenic inheritance of apomixis in two Hieracium species with distinct developmental mechanisms. Heredity 84:228–237
Bradford K, and Nonagaki H (Eds.) (2007) Seed development, dormancy, and germination. Annual Plant Review Vol. 27. Blackwell Pub., Oxford, U.K
Brukhin V (2017) Molecular and genetic regulation of apomixis. Russ J Genet 53:943–964
Carman JG, Crane CF, Hughes JE (1985) Hybrid crops that clone themselves. Utah Sci 46:90–94
Chen B, Maas L, Figueiredo D, Zhong Y, Reis R, Li M, Horstman A, Riksen T, Weemen M, Liu H, Siemons C, Chen S, Angenent GC, Boutilier K (2022) BABYBOOM regulates early embryo and endosperm development. Proc Natl Acad Sci 119(25):2201761119. https://doi.org/10.1073/pnas.2201761119
Conner JA, Ozias-Akins P (2017) Apomixis: engineering the ability to harness hybrid vigor in crop plants. Methods Mol Biol 1669:17–34
Fedorov AA (1974) Chromosome numbers of flowering plants. Academy of Sciences, Leningrad
Fedorov A.A. (1969). Chromosome numbers of flowering plants. Leningrad: Academy of Sciences of the USSR, the Komarov VL Botanical Institute, Nauka, Leningrad
Folsom JJ, Begcy K, Hao X, Wang D, Walia H (2014) Rice fertilization- Independent Endosperm1 regulates seed size under heat stress by controlling early endosperm development. Plant Physiol 165(1):238–248
Hand ML (2015) Evolution of apomixis loci in Pilosella and Hieracium (Asteraceae) inferred from the conservation of apomixis-linked markers in natural and experimental populations. Heredity (Edinb) 114(1):17–26
Hand ML, Koltunow AMG (2014) The genetic control of apomixis: asexual seed formation. Genetics 197:441–450
Herr JM (1971) A new clearing-squash technique for the study of ovule development in angiosperms. Am J Bot 58:760–795
Herr JM (1974) A clearing-squash technique for the study of ovule and megagametophyte development in angiosperms. In: Radford AE, Dickinson WC, Massey JR, Bell CR (eds) Vascular Plant Systematics. Harper and Rowe, New York, pp 230–235
Hyde L, Osman K, Winfield M, Sanchez-Moran E, Higgins JD, Henderson IR, Sparks C, Franklin FCH, Edwards KJ (2022) Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants. Plant Biotechnol J. https://doi.org/10.1111/pbi.13961
Khanday I, Skinner D, Yang B, Mercier R, Sundaresan V (2019) A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds. Nature 565:91–95
Kirchheimer B (2018) Reconstructing geographical parthenogenesis: effect of niche differentiation and reproductive mode on Holocene range expansion of an alpine plant. Ecol Lett 21:392–401
Klatt S, Schinkel CCF, Kirchheimer B, Dullinger S, Horandl E (2018) Effects of cold treatments on fitness and mode of reproduction in the diploid and polyploid alpine plant Ranunculus kuepferi (Ranunculaceae). Ann Bot 121:1287–1298
Koltunow AM (1993) Apomixis: embryo sacs and emryos formed without meiosis or fertilization in ovules. Plant Cell 5:1425–1437
Koltunow AM, Bicknell RA, Chaudhury AM (1995) Apomixis: Molecular strategies for the generation of genetically identical seeds without fertilization. Plant Physiol 108:1345–1352
Kreiner JM, Kron P, Husband BC (2017) Evolutionary dynamics of unreduced gametes. Trends Genet 33:583–593
Maia FR, Varassin IG, Goldenberg R (2016) Apomixis does not affect visitation to flowers of Melastomataceae, but pollen sterility does. Plant Biol 18(1):132–138
Mieulet D, Jolivet S, Rivard M, Cromer L, Vernet A et al (2016) Turning rice meiosis into mitosis. Cell Res 26:1242–1254. https://doi.org/10.1038/cr.2016.117
Nogler GA (1982) How to obtain diploid apomictic Ranunculus auricomus plants not found in the wild state. Bot Helvet 92:13–22
Nogler GA (1984) Gametophytic apomixis. In: Johri BM (ed) Embryology of Angisoperms. Springer, Berlin, pp 475–518
Ogawa D, Johnson SD, Henderson ST, Koltunow AMG (2013) Genetic separation of autonomous endosperm formation (AutE) from the two other components of apomixis in Hieracium. Plant Reprod 26(2):113–123. https://doi.org/10.1007/s00497-013-0214-y
Ozias-Akins P, van Dijk PJ (2007) Mendelian genetics of apomixis in plants. Ann Rev Genet 41:509–537
Ozias-Akins P, Roche D, Hanna WW (1998) Tight clustering and hemizygosity of apomixis-linkedmolecular markers in Pennisetum squamulatumimpliesgenetic control of apospory by a divergent locus thatmay have no allelic form in sexual genotypes. Proc Natl Acad Sci USA 95:5127–5132
Pupilli F, Barcaccia G (2012) Cloning plants by seeds: inheritance models and candidates genes to increase fundamental knowledge for engineering apomixis in sexual crops. J Biotechnol 159(4):291–311
Richards AJ (2003) Apomixis in flowering plants: an overview. Philos Tran R Soc Lond B Biol Sci 358:1085–1093
RoshevitsRYu, (1980) Grasses: An introduction to the study of fodder and cereal grasses. Indian National Scientific Documentation Center, New Delhi 6:635
Rychlewski J (1967) Karyological studies on Nardusstricta L. Acta Biol Cracov Ser Botan 10:55
Savidan Y (2000a) Apomixis: genetics and breeding. Plant Breed Rev 18:13–86
Savidan Y, Carman J.G. and Dresselhaust T. (2001). The Flowering of Apomixis: from Mechanisms to Genetic Engineering. Mexico, DF: CIMMYT, IRD, European Commission DG VI (FAIR)
Savidan Y (2000a) Apomixis, the way of cloning seeds. Biofutur, pp. 38–43
Savidan Y (2001) Gametophytic Apomixis. In: Current Trends in the Embryology of Angiosperms, eds. Bhojwani SS, Soh WY. Netherlands: Springer Dordrecht. pp. 419−33. https://doi.org/10.1007/978-94-017-1203-3_16
Schwartz YB, Pirrotta V (2008) Polycomb complexes and epigenetic states. Curr Opin Cell Biol 20(3):266–273
Spillane C, Curtis MD, Grossniklaus U (2004) Apomixis technology development-virgin births in farmers’ fields? Nat Biotechnol 22:687–691. https://doi.org/10.1038/nbt976
Srivastava AK (1982) Apomixis in the angiosperm. Acta Bot Indica 10:111–113
Srivastava AK, Purnima (1990) Numerical and structural inconstancy in Belamchanda chinensis DC (Iridaceae). Proc Indian Acad Sci 100:205–210
Tucker MR (2003) Sexual and apomictic reproduction in Hieracium subgenus Pilosella are closely interrelated developmental pathways. Plant Cell Online 15(7):1524–1537
Ulukan H (2009) The evolution of cultivated plant species: Classical plant breeding versus genetic engineering. Plant Syst Evolu 280:133–142
Van Dijk PJ, Op den Camp R, Schauer SE (2020) Genetic dissection of Apomixis in dandelions identifies a dominant parthenogenesis locus and highlights the complexity of autonomous endosperm formation. Genes 11(9):961. https://doi.org/10.3390/genes11090961
Vijverberg K, Milanovic-Ivanovic S, Bakx-Schotman T, van Dijk PJ (2010) Genetic fine-mapping of DIPLOSPOROUS in Taraxacum (dandelion; Asteraceae) indicates a duplicated DIP-gene. BMC Plant Biol 10:154. https://doi.org/10.1186/1471-2229-10-154
Wang C, Liu Q, Shen Y, Hua Y, Wang J et al (2019) Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes. Nat Biotechnol 37:283–286. https://doi.org/10.1038/s41587-018-0003-0
Warmke HE (1954) Apomixis in Panicum maximum. Am J Bot 41:5–10
Author Information
Department of Botany, Multanimal Modi College, Modinagar, India