Vegetos
(An International Journal of Plant Research & Biotechnology)
Detection of Bacillus cereus from food samples on portable culture device
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Research Articles | Published: 04 September, 2024
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Keywords: On-site detection, Foodborne pathogens, n Bacillus cereusn , Point of care testing (POCT), Portable culture device (PCD)
Abstract
The incidence of foodborne illnesses is increasing in countries with poor infrastructure and limited access to health care services. The reliance on advanced laboratory facilities and highly trained professionals restricts the adoption of modern testing techniques, particularly in remote areas, highlighting the necessity for on-site cost-effective testing methods such as Portable Culture Devices (PCD). This study aimed to standardize the use of PCDs to detect microorganisms in food samples. Bacillus cereus was successfully detected using enzyme-chromogenic substrate interactions. The device, optimized at a substrate concentration of 3 gL−1 and a temperature range of 28–37 °C, can detect cell counts of up to 10 CFU/mL. Proof-of-concept tests on spiked food samples, as well as on-site laboratory-independent detection, demonstrate the practicality of PCDs as an alternative to traditional culture-dependent detection methods. Their affordability, ease of use, and minimal sample requirements make them suitable for point-of-care testing in resource-constrained settings, and meet the criteria for REASSURED detection devices.
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Author Information
Department of Microbiology, Ramnarain Ruia Autonomous College, Matunga, India