Cryopreservation of shoot meristems of Baliospermum montanum (Willd.) Muell. Arg. for long-term conservation and sustainable utilization

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Doi: 10.1007/s42535-023-00720-3
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Keywords: n Baliospermum montanumn , Cryopreservation, Genetic stability, ISSR markers, PVS2, Vitrification


Abstract


Long-term in vitro conservation through cryopreservation was successfully established for the first time in Baliospermum montanum (Willd.) Muell. Arg. (Euphorbiaceae), an important medicinal plant in Southern Western Ghats. The aseptically dissected shoot apical meristems of B. montanum after preculture in sucrose (0.4 M) enriched Murashige and Skoog (MS) medium followed by loading solution treatment for 20 min and upon subsequent dehydration with Plant Vitrification Solution (PVS2) for 60 min produced 63.33% recovery after liquid nitrogen exposure. The cryopreserved meristems regenerated in to healthy plantlets in MS medium augmented with 1.0 mgl− 1 6-benzyl amino purine (BAP) and 0.1 mgl− 1 Indole 3-acetic acid (IAA). Furthermore, molecular analysis using ISSR (Inter Simple Sequence Repeat) markers revealed monomorphic banding pattern and did not show any polymorphism among the mother plant and cryopreserved plantlets. The findings are suggesting that cryopreservation via vitrification does not affect the genetic stability in B. montanum and it can be considered as a promising method for long-term conservation of this genetic resource.


n              Baliospermum montanumn            , Cryopreservation, Genetic stability, ISSR markers, PVS2, Vitrification


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Acknowledgements



Author Information


Parvathi K. P.
Plant Tissue Culture Laboratory, Department of Botany, University College, Research Centre, University of Kerala, Thiruvananthapuram, India

Preetha T. S.
Plant Tissue Culture Laboratory, Department of Botany, University College, Research Centre, University of Kerala, Thiruvananthapuram, India
preethahemanth@yahoo.com

Hemanthakumar A. S.
Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden & Research Institute, Palode, Thiruvananthapuram, India