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Keywords:
Antifungal activity, Crude extracts and secondary metabolites, Disc diffusion assay, Minimum inhibitory concentration, Total activity
Candida albicans is the most significant species of opportunistic fungus that causes infections in immunocompromised people with invasive fungal illnesses. Scientists are now paying more attention to herbs used in traditional medicine as a result of Candida species’ resistance to antifungal medications. This study was conducted to compare the antifungal impacts of herbal leaf extracts of Carissa carandas Linn., Nerium oleander Linn., and Allamanda cathartica Linn. due to the limitations in the treatment of fungal diseases, like high prices, adverse side effects, drug resistance, or reduced susceptibility to fungal drugs. Leaves were collected, dried, and extracted using standard techniques for alkaloids, flavonoids, steroids, and crude extracts in petroleum ether, methanol, and water, then tested for their antifungal efficacy. A disc diffusion assay was used for screening, and the inhibition zone and activity index were calculated. The broth micro dilution method was used to calculate the minimum inhibitory concentration and the minimum fungicidal concentration. Total activity, mean, and standard deviation were also calculated. Alkaloid extracts of A. cathartica Linn. showed the best activity (IZ = 22 mm, AI = 0.650.01, MIC = 0.078, MFC = 0.156 mg/mL, TA = 557.69 mL). Results showed that among all the examined leaf extracts from the three selected plants, the alkaloid extract of A. cathartica Linn. leaves was the most efficient against Candida albicans. As a result, this plant may be investigated for the development of novel and improved herbal antifungal medications.
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The author is thankful to the Head of the Department of Botany, University of Rajasthan, for providing all necessary facilities and to Prof. Padma Kumar for providing language help, writing assistance, and proofreading the article.