Chlorophyll fluorescence and antioxidants activity as influenced by zinc nutrition in wheat under late sown conditions

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00552-7
First Page: 1509
Last Page: 1516
Views: 370


Keywords: Zinc, Wheat, Late sowing, Heat stress, Chlorophyll fluorescence, Antioxidants


Abstract


Delayed sowing has grievous effect on the yield in wheat crop due to high temperature at grain filling stage leading to terminal heat stress. This field experiment was conducted to discern the impact of different modes of ZnSO4 application in mitigating delayed sowing induced heat stress in wheat varieties through analyzing chlorophyll fluorescence and antioxidants activity. A field experiment was laid out with three varieties of wheat viz., UP 2425 (V1), UP 2565 (V2) and HD 3059 (V3) sown at late sown conditions. Four zinc treatments viz., T1: soil ZnSO4 application @ 25 kg ha− 1, T2: foliar ZnSO4 spray (0.5%), T3: soil ZnSO4 application @ 25 kg ha− 1 + foliar ZnSO4 spray (0.5%) and a control treatment without any ZnSO4 were applied at tillering, panicle emergence and grain filling stages. Chlorophyll fluorescence and antioxidants activity were recorded at panicle emergence and grain filling stages along with grain yield after harvesting. All the zinc treatments improved grain yield, chlorophyll fluorescence, proline content, superoxide dismutase (SOD) and glutathione reductase (GR) activity compared to control in the late sown wheat crop. Among all the ZnSO4 treatments, foliar and foliar + soil treatments were found more effective in mitigating heat stress followed by soil treatment alone. The findings of this study divulged that zinc nutrition imparted heat tolerance and augmented grain yield in late sown wheat owing to enhanced antioxidants activity which protected chloroplast membranes and photosynthetic apparatus from heat stress damage implied by delayed sowing.


Zinc, Wheat, Late sowing, Heat stress, Chlorophyll fluorescence, Antioxidants


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References


Almeselmani M, Deshmukh PS, Chinnusamy V (2012) Effect of prolonged high temperature stress on respiration, photosynthesis and gene expression in wheat (Triticum aestivum L.) varieties differing in their thermotolerance. Plant stress 6:25–32


Aslam MA, Ahmed M, Stockle CO, Higgins SS, Hayat R (2017) Can growing degree days and photoperiod predict spring wheat phenology. Front Environ Sci 5:57–68


Asseng S, Ewert F, Martre P, Rotter RP, Lobell DB, Cammarano D, Zhu A (2015) Rising temperatures reduce global wheat production. Nat Clim Change 5:143–147


Asthir B (2015) Mechanisms of heat tolerance in crop plants. Biol Plant 59:620–628


Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207


Bergkamp B, Impa SM, Asebedo AR, Fritz AK, Jagadish SVK (2018) Prominent winter wheat varieties response to post flowering heat stress under controlled chambers and field based heat tents. Field crops Res 222:143–152


Cakmak I (2000) Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146:185–205


Cousins RJ (1998) Role of zinc in regulation of gene expression. Proceed Nut Soc 57:307–311


Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25


Haque MS, Kjaer KH, Rosenqvist E, Sharma DK, Ottosen CO (2014) Heat stress and recovery of photosystem II efficiency in wheat (Triticum aestivum L.) cultivars acclimated to different growth temperatures. Environ Exp Bot 99:1–8


Hasanuzzaman B, Bhuyan MHMB, Anee TI, Parvin K, Nahar K, Al Mahmud J, Fujita M (2019) Regulation of ascorbate–glutathione pathway in mitigating oxidative damage in plants under abiotic stress. Antioxidants 8:384


Hera MHR, Hossain M, Paul AK (2018) Effect of foliar zinc spray on growth and yield of heat tolerant wheat under water stress. Int J Biological Env Eng 1(1):10–16


Hossain MM, Hossain A, Alam MA, Sabagh EL, Haque A, Muriruzzaman MM, Islam M, Das MZ, Barutcular S, Kizilgeci C F (2018) Evaluation of fifty irrigated spring wheat genotypes grown under heat stress conditions in multiple environments of Bangladesh. Fresenius Environ Bull 27:5993–6004


Hussain HA, Men S, Hussain S, Chen Y, Ali S, Zhang S, Zhang K, Li Y, Xu Q, Liao C (2019) Interactive effects of drought and heat stresses on morpho-physiological attributes, yield, nutrient uptake and oxidative status in maize hybrids. Sci Rep 9:3890


Iqbal MA, Imtiaz H, Muzammil HS, Essa A, Zahoor A (2018) Probing profitability of irrigated and rainfed bread wheat (Triticum aestivum L.) crops under foliage applied sorghum and moringa extracts in Pakistan. Custos e agronegocio 14:2–16


Joshi AK, Chand R, Arun B, Singh RP, Ortiz R (2007) Breeding crops for reduced-tillage management in the intensive rice–wheat systems of South Asia. Euphytica 153:135–151


Kakkar P, Das B, Viswanathan NP (1984) A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21:130–132


Kandoliya RU, Sakarvadiya HL, Kunjadia BB (2018) Effect of zinc and iron application on leaf chlorophyll, carotenoid, grain yield and quality of wheat in calcareous soil of Saurashtra region. Int J Chem Studies 6(4):2092–2096


Kocsy G, Szalai G, Galiba G (2002) Effect of heat stress on glutathione biosynthesis in wheat. Acta Biologica Szegediensis 46(3–4):71–72


Lalelou FS, Fateh M (2014) Effects of different concentrations of zinc on chlorophyll, starch, soluble sugars and proline content in Cucurbita pepo. Inter J Biosci 4:6–12


Liu Y, Xu C, Zhu Y, Zhang L, Chen T, Zhou F, Chen H, Lin Y (2018) The calcium dependent kinase OsCPK24 functions in cold stress response in rice. J Integr Plant Biol 60:173–188


Luo H, Bin D, He L, He J, Hu L, Pan S, Tang X (2019) Exogenous application of zinc (zn) at the heading stage regulates 2-acetyl-1-pyrroline (2-AP) biosynthesis in different fragrant rice genotypes. Sci Rep 9:19513


Ma D, Sun D, Wang C, Ding H, Qin H, Hou J, Huang X, Xie Y, Guo T (2017) Physiological responses and yield of wheat plants in zinc-mediated alleviation of drought stress. Front Plant Sci 8:860


Michael PI, Krishnaswamy M (2014) Membrane damage and activity of antioxidant enzymes in response to zinc and high irradiance stress in cowpea plant. Int J Curr Res Acad Rev 2:112–128


Moghadam HRT, Zahedi H, Ashkiani A (2013) Effect of zinc foliar application on auxin and gibberellin hormones and catalase and superoxide dismutase enzyme activity of corn (Zea mays L.) under water stress. Maydica 58:218–223


Mohi-Ud-Din M, Siddiqui N, Rohman M, Jagadish SVK, Ahmed JU, Hassan MM, Hossain A, Islam T (2021) Physiological and biochemical dissection reveals a trade-off between antioxidant capacity and heat tolerance in bread wheat (Triticum aestivum L.). Antioxidants 10:351


Nawaz A, Farooq M, Nadeem F, Siddique KHM, Lal R (2019) Rice-wheat cropping systems in South Asia: issues, options and opportunities. Crop Past Sci 70:395–427


Osredkar J, Sustar N (2011) Copper and zinc, biological role and significance of copper/zinc imbalance. J Clin Toxicol 3:001


Peck AW, McDonald GK (2010) Adequate zinc nutrition alleviates the adverse effects of heat stress in bread wheat. Plant Soil 337:355–374


Rehman SU, Bilal M, Rana RM, Tahir MN, Shah MKN, Ayalew H, Yan G (2016) Cell membrane stability and chlorophyll content variation in wheat (Triticum aestivum) genotypes under conditions of heat and drought. Crop Pasture Sci 67:712–718


Ristic Z, Bukovnik U, Prasad PV (2007) Correlation between heat stability of thylakoid membranes and loss of chlorophyll in winter wheat under heat stress. Crop Sci 47:2067–2073


Sadeghzadeh B (2013) A review of zinc nutrition and plant breeding. J Soil Sci Plant Nut 13:905–927


Sarafraz-Ardakani MR, Khavari-Nejad RA, Moradi F, Najafi F (2014) Abscisic acid and cytokinin induced osmotic and antioxidant regulation in two drought tolerant and drought sensitive cultivars of wheat during grain filling under water deficit in field conditions. Not Sci Biol 6:354–362


Schittenhelm S, Langamp-Wedde T, Kraft M, Kottmann L, Matschiner K (2020) Effect of two week heat stress during grain filling on stem reserves, senescence and grain yield of european winter wheat cultivars. J Agro Crop Sci 206:722–733


Sharma D, Singh R, Tiwari R, Kumar R, Gupta V (2019) Wheat responses and tolerance to terminal heat stress: a review. In: Hasanuzzaman M, Nahar K, Hossain MA (eds), Wheat production in changing environments: responses, adaptation and tolerance, pp 149–173


Sofy MR (2015) Application of salicylic acid and zinc improves wheat yield through physiological processes under different levels of irrigation intervals. Int J Plant Res 5:136–156


Suganya A, Saravanan A, Maivannan N (2020) Role of zinc nutrition for increasing zinc availability, uptake, yield and quality of maize (Zea mays L.) grains: an overview. Comm Soil Sci Plant Anal 51:2001–20021


Tavallali V, Rahemi M, Eshghi S, Kholdebarin B, Ramezanian A (2010) Zinc alleviates salt stress and increases antioxidant enzyme activity in the leaves of pistachio (Pistacia vera L ‘Badami’) seedlings. Turkish J Agric Forestry 34:349–359


Tiwari YK, Yadav SK (2019) Effect of high temperature stress on ascorbate-glutathione cycle in maize. Agric Res 9:179–187


Ullah S, Samreen T, Shah HU, Javid M (2017) Zinc effect on growth rate, chlorophyll, protein and mineral contents of hydroponically grown mungbeans plant (Vigna radiata). Arab J Chem 10:1802–1807


Ullah A, Romdhane L, Rehman A, Farooq M (2019) Adequate zinc nutrition improves the tolerance against drought and heat stresses in chickpea. Plant Physiol Biochem 143:11–18


Wang H, Liu RL, Jin JY (2009) Effects of zinc and soil moisture on photosynthetic rate and chlorophyll fluorescence parameters of maize. Biol Plant 53:191–194


Yildirim M, Barutcular C, Hossain A, Koc M, Dizlek H, Akinci C, Toptas I, Basdemir F, Islam MS, Sabagh EL (2018) Assessment of the grain quality of wheat genotypes grown under multiple environments using GGE iplot analysis–fresenius. Environ Bull 27:4830–4837


Zafar S, Nasri M, Moghadam HRT, Zahedi H (2014) Effect of zinc and sulfur foliar applications on physiological characteristics of sunflower (Helianthus annuus L.) under water deficit stress. Int J Biosci 5:87–96

 


Acknowledgements


we thank Dr. V.P. Singh, Professor, Dept. of Agronomy, GBPUAT, Pantnagar for providing land, seed material, fertilizers and labor for the experiment at Norman E. Borlaug crop research centre, Pantnagar.


Author Information


Chatti Dheeraj
Department of Plant Physiology, GBPUAT, Pantnagar, India
chattidheeraj@gmail.com
Kumar Atul
Department of Plant Physiology, GBPUAT, Pantnagar, India
atulvkumar@gmail.com