Vegetos
(An International Journal of Plant Research & Biotechnology)
Chemical identification, compositional evaluation, proximate analysis and assessment of storage proteins of Telfairia occidentalis (Hook f.) seed protein isolate
*Article not assigned to an issue yet
Ruth Olasehinde Oluwaseun, Bamikole Afolabi Olakunle, Olusanya Arise Rotimi
Research Articles | Published: 28 August, 2023
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
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Keywords:
SDS-PAGE, Protein hydrolysate, Seed flour, GC–MS analysis, Protein fractionation
Abstract
Over the past few decades, there has been an increase in the industrial use of plant protein isolates as functional ingredients to enhance the nutritional quality of various products. Thus, this study aimed to evaluate the composition, proximate analysis, storage proteins, and compound identification of T. occidentalis seed protein isolate (TOSPI). Protein was extracted from the defatted T. occidentalis seeds using acid and isoelectric precipitation methods. The proximate analysis was carried out using standard procedures, while compositional evaluation and compound identification were performed with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and gas chromatography-mass spectrometry (GC–MS) techniques. The results of the proximate analysis showed that the protein content of undefatted seeds, defatted seeds, and TOSPI was 36.86%, 46.17%, and 86.50%, respectively. Protein fractionation of TOSPI revealed the presence of albumin (17.72 ± 0.16 mg dL−1), globulin (10.57 ± 0.22 mg dL−1), prolamin (12.81 ± 0.08 mg dL−1), and glutenin (9.89 ± 0.12 mg dL−1). The SDS-PAGE patterns observed in the defatted seeds and TOSPI of T. occidentalis indicated that the most prominent bands were located in the molecular weight range of approximately 20 and 30 kDa, respectively. Additionally, GC–MS analysis identified the presence of lectin, vicilin CG-72, glycinin, β-conglycinin, oleosin, kunitz trypsin inhibitor, histone H2B, gluten, and legumin B. Therefore, considering these properties, T. occidentalis seed protein isolate indicates a promising source of materials for the pharmaceutical and industrial applications.
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Acknowledgements
The authors hereby appreciate the management of the Afe Babalola University where the larger part of the work was performed and for creating an enabling environment for the attainment of this research study.
Author Information
Medical Biochemistry Unit, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria
Department of Chemical Sciences, Biochemistry Programme, College of Science, Afe Babalola University, Ado-Ekiti, Nigeria
afolabiob@abuad.edu.ng