Cadmium biosorption and plant growth promotion efficacy of a metalloresistant Pseudomonas sp. unveils augmented growth with reduced metal accumulation in Brassica napus L.

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00724-z
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Keywords: Biosorption, Adsorption isotherms, n Pseudomonas aeruginosan , Plant growth promotion, Bioreduction


Abstract


Heavy metal-resistant plant growth-promoting bacteria may help in reducing the toxic metal accumulation within the plants grown in metal-contaminated soils and thus be effective against biomagnification, enhancing food safety. In the present study, the most potential multi-metal resistant bacterium Pseudomonas aeruginosa JCM 5962 (obtained from arable soil of Uttar Dinajpur) was utilized for Cd biosorption analysis and also to observe the in vivo growth effect on Brassica plant including the estimation of Cd content accumulated within the plant parts of Brassica grown in Cd-contaminated soil. The maximum sorption of Cd ions by the dead bacterial biomass of Pseudomonas aeruginosa JCM 5962 was estimated to be 149.25 mg/g. The adsorption data provided an outstanding fit to the Freundlich isotherm model, indicating multilayer adsorption at heterogeneous surfaces. RL value (0.76, i.e.; <1) implied favourable adsorption. The in vivo experiment revealed the increased length of both roots and shoots in Brassica seedlings grown in Cd-contaminated soil under the effect of bacterial inoculants, which was also statistically significant (p < 0.001). In addition, the studied bacterium was effective in lowering Cd accumulation within the plant parts of Brassica. Approximately 62.76% reduction in Cd content was observed in Brassica seedlings grown in Cd-contaminated soil under the effect of bacterial inoculants than in the non-inoculated control set. This is a pioneering study, which could be further employed to develop strategies for microbe-assisted phytoremediation.


Biosorption, Adsorption isotherms, n              Pseudomonas aeruginosan            , Plant growth promotion, Bioreduction


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Acknowledgements


The authors would like to acknowledge DST-SAIF of IIT-Bombay for providing sophisticated analytical services (ICP-AES and ICP-MS). The authors also thank Mr. Vivek Roy, Mr. Barnan K. Saha, Ms. Samarpita Adhikary, and Mr. Prajesh Dutta for their support and cooperation.


Author Information


Saha Jayanti
Department of Botany, Acharya Prafulla Chandra Roy Govt. College, Himachal Vihar, Matigara, Siliguri, India
jayantisaha98@gmail.com
Pal Ayon
Microbiology and Computational Biology Laboratory, Department of Botany, Raiganj University, Raiganj, India