Assessment of phytometabolite distribution, in vitro antioxidant and anti-inflammatory potential of novel plant, Euphorbia deccanensis—endemic to South India

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
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Doi: 10.1007/s42535-023-00700-7
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Keywords: Phytometabolites, Antioxidant and anti-inflammatory activity


Abstract


Chronic diseases, such as different cancers, are primarily caused by inflammation and oxidative stress. Due to their prominent roles, it is believed that oxidative stress and inflammation reduction should be a goal for preventing these disorders. Numerous natural products and plant extracts offer defense against free radicals and damage caused by them. The novel plant E. deccanensis, which is an endemic to South India, was investigated in this study to evaluate the distribution of phytochemicals, quantitative estimation of phenolics and flavonoids, and to analyze its anti-inflammatory and antioxidant potential. Phytochemical screening revealed the presence of powerful compounds such as flavonoids, phenolics, coumarins, terpenoids, triterpenoids, etc. Total phenolic content was determined to be 139 ± 2.51 mg GA/g DW and total flavonoid content to be 59.3 ± 3.3 mg QE/g DW. Plant extract exhibited a significant antioxidant potential against all the free radicals chosen in a dose-dependent manner. Additionally, signs of anti-inflammatory properties include inhibition of major enzymes which are involved in inflammation such as cyclooxygenase, lipoxygenase, and inducible nitric oxide synthase, as well as drop-in nitrite levels. ELISA results, which demonstrated the plant extracts incredible capacity to significantly reduce the level of COX-2, one of the key enzymes involved in inflammation, supported this as well. This study also justify the use of this plant by the tribes of Tirunelveli Hills, India, for the treatment of various diseases. The plants flavonoids, phenolics, and terpenoids suggest that it could serve as a starting point for the development of novel therapeutics for a range of diseases.


Phytometabolites, Antioxidant and anti-inflammatory activity


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References


Amri O, Zekhnini A, Bouhaimi A, Tahrouch S, Hatimi A (2018) Anti-inflammatory activity of methanolic extract from Pistacia atlantica Desf. leaves. Pharmacognosy J 10(1):71–76


Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N (2019) Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants 8(4):96


Asanuma M, Nishibayashi-Asanuma S, Miyazaki I, Kohno M, Ogawa N (2001) Neuroprotective effects of non-steroidal anti-inflammatory drugs by direct scavenging of nitric oxide radicals. J Neurochem 76(6):1895–1904


Axelrod B, Cheesbrough TM, Laakso S (1981) Lipoxygenase from soybeans: EC 1.13. 11.12 Linoleate: oxygen oxidoreductase. Methods Enzymol Academic Press 71:441–451


Bai J, Zhang Y, Tang C, Hou Y, Ai X, Chen X, Meng X (2021) Gallic acid: Pharmacological activities and molecular mechanisms involved in inflammation-related diseases. Biomed Pharmacother 133:110985


Balaji S, Ganesan KK (2021) Inhibition of inducible nitric oxide production by Caryota urens and its active constituents umbelliferone and rutin. J Ayurveda Integr Med 12(2):369–372


Barka ZB, Lahbib K, Aouadhi C, Ladjimi MH, Miled HB, Rhouma KB, Tebourbi O (2019) Antioxidant, Antimicrobial and Anti-Inflammatory Activities of Edible Rhus tripartita (Ucria) Hydromethanolic Extracts. J Pharm Pharmacol Res 3(3):74–87


Ben-Shabat S, Yarmolinsky L, Porat D, Dahan A (2020) Antiviral effect of phytochemicals from medicinal plants: applications and drug delivery strategies. DDTR 10(2):354–367


Borquaye LS, Laryea MK, Gasu EN, Boateng MA, Baffour PK, Kyeremateng A (2020) Anti-inflammatory and antioxidant activities of extracts of Reissantia indica, Cissus cornifolia and Grosseria vignei. Cogent Biol 6(1):1785755


Cappell MS, Schein JR (2020) Diagnosis and treatment of nonsteroidal anti-inflammatory drug-associated upper gastrointestinal toxicity. Gastroenterol Clin North Am 29(1):97–124


Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF (2009) Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem 49(7):3420–3424


Chen CH, Lin CH, Shih CK (2021) Antioxidant activity of plant extracts evaluated by DPPH radical scavenging assay. Antioxidants 10(3):397


Diab TA, Donia T, Saad-Allah KM (2021) Characterization, antioxidant, and cytotoxic effects of some Egyptian wild plant extracts. Beni-Suef Univ J Basic Appl Sci 10(1):1–13


Gallorini M, Rapino M, Schweikl H, Cataldi A, Amoroso R, Maccallini C (2021) Selective inhibitors of the inducible nitric oxide synthase as modulators of cell responses in LPS-stimulated human monocytes. Molecules 26(15):4419


Kawra M, Saklani S, Parcha V (2020) Preliminary phytochemical screening and antioxidant activity of five medicinal plants of garhwal himalaya: a comparitive study. Vegetos 33(3):610–613


Ketha A, Vedula GS, Sastry AVS (2020) In vitro antioxidant, anti-inflammatory, and anticancer activities of methanolic extract and its metabolites of whole plant Cardiospermum canescens Wall. Fut J Pharm Sci 6(1):1–10


Kim DJ, Lee MH, Liu K, Lim DY, Roh E, Chen H, Dong Z (2017) Herbacetin suppresses cutaneous squamous cell carcinoma and melanoma cell growth by targeting AKT and ODC. Carcinogenesis 38(11):1136–1146


Kumar GS, Jayaveera KN, Kumar CK, Sanjay UP, Swamy BM, Kumar DV (2007) Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Trop J Pharm Res 6(2):717–723


Kumaran A, Joel Karunakaran R (2006) Antioxidant activities of the methanol extract of Cardiospermum halicacabum. Pharm Biol 44(2):146–151


Kumari S, Deori M, Elancheran R, Kotoky J, Devi R (2016) In vitro and in vivo antioxidant, anti-hyperlipidemic properties and chemical characterization of Centella asiatica (L.) extract. Front Pharmacol 7:400


Kunchandy E, Rao MNA (1990) Oxygen radical scavenging activity of curcumin. Int J Pharm 58(3):237–240


Kuwano T, Nakao S, Yamamoto H, Tsuneyoshi M, Yamamoto T, Kuwano M, Ono M (2004) Cyclooxygenase 2 is a key enzyme for inflammatory cytokine-induced angiogenesis. FASEB J 18(2):300–310


Lepoivre M, Chenais B, Yapo A, Lemaire G, Thelander L, Tenu JP (1990) Alterations of ribonucleotide reductase activity following induction of the nitrite-generating pathway in adenocarcinoma cells. JBC 265(24):14143–14149


Liu J, Yu W, Sun L, Lu W, Li H, Liu C, Wei H (2021) Anti-inflammatory effect of red ginseng oil in lipopolysaccharide-stimulated RAW264.7 cells: Regulation of COX-2/NF-κB signaling pathway. Front. Pharmacol 12:628074


Loganayaki N, Siddhuraju P, Manian S (2013) Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L. J Food Sci Technol 50:687–695


Maleki SJ, Crespo JF, Cabanillas B (2019) Anti-inflammatory effects of flavonoids. Food Chem 299:125–124


Maqsood M, Qureshi R, Ikram M, Ahmad MS, Jabeen B, Asi MR, Lilge L (2018) In vitro anticancer activities of Withania coagulans against HeLa, MCF-7, RD, RG2, and INS-1 cancer cells and phytochemical analysis. Integrat Med Res 7(2):184–191


Mo YN, Cheng F, Yang Z, Shang XF, Liang JP, Shang RF, Liu Y (2018) Antioxidant activity and the potential mechanism of the fruit from Ailanthus altissima Swingle. Front Vet Sci 8:784898


Nunes CDR, Barreto Arantes M, de Faria Menezes, Pereira S, Leandro da Cruz L, de Souza Passos M, Pereira de Moraes L, Barros de Oliveira D (2020) Plants as sources of anti-inflammatory agents. Molecules 25(16):3726


Oueslati S, Ksouri R, Falleh H, Pichette A, Abdelly C, Legault J (2012) Phenolic content, antioxidant, anti-inflammatory and anticancer activities of the edible halophyte Suaeda fruticosa Forssk. Food Chem 132(2):943–947


Pandey BP, Adhikari K, Pradhan SP, Shin HJ, Lee EK, Jung HJ (2020) In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal. FJPS 6(1):1–12


Preethamol SN, Thoppil JE (2020) Phenolic content, flavonoid content and antioxidant potential of whole plant extract of Ophiorrhiza pectinata Arn. Indian J Pharm Sci 82(4):712–718


Recchiuti A, Isopi E, Romano M, Mattoscio D (2020) Roles of specialized pro-resolving lipid mediators in autophagy and inflammation. Int J Mol Sci 18:6637


Salter M, Duffy C, Garthwaite J, Strijbos PJ (1996) Ex vivo measurement of brain tissue nitrite and nitrate accurately reflects nitric oxide synthase activity in vivo. J Neurochem 66(4):1683–1690


Sameena VP, Thoppil JE (2023) Invitro antibacterial efficacy of methanolic plant extract and biosynthesized silver nanoparticles from Euphorbia species against two selected foodborne pathogens. J Nanotechnol Environm Eng 2:13


Serhan CN, Levy BD (2018) Resolvins in inflammation: emergence of the pro-resolving superfamily of mediators. J Clin Investig 128(7):2657–2669


Shaikh RU, Pund MM, Gacche RN (2016) Evaluation of anti-inflammatory activity of selected medicinal plants used in Indian traditional medication system in vitro as well as in vivo. J Tradit Complement Med 6(4):355–361


Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, Dini L, Panzarini E, Sharifi-Rad J (2020) Lifestyle, oxidative stress, and antioxidants: Back and forth in the pathophysiology of chronic diseases. Front Physiol 11:694


Sousa F, Guebitz GM, Kokol V (2009) Antimicrobial and antioxidant properties of chitosan enzymatically functionalized with flavonoids. Process Biochem 44(7):749–756

 


Acknowledgements


The authors are thankful for the taxonomic identification of the plant specimens by Dr. A. K. Pradeep, Assistant Professor, Department of Botany, University of Calicut. This work was funded by the Council of Scientific and Industrial Research, New Delhi.


Author Information


Sameena V. P.
Cell and Molecular Biology Division, Department of Botany, University of Calicut, Kerala, India
Sameenavvp95@gmail.com
Thoppil J. E.
Cell and Molecular Biology Division, Department of Botany, University of Calicut, Kerala, India
jethoppil@gmail.com