Vegetos
(An International Journal of Plant Research & Biotechnology)
Antimicrobial potential of in vitro and in vivo plant parts of Paeonia emodi Wallich Ex Royle (Himalayan peony)
Research Articles | Published: 18 June, 2022
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
First Page: 494
Last Page: 505
Views: 570
Keywords:
Paeonia emodi
, Callus, Minimum inhibitory concentration (MIC), Solvent, Altitude
Abstract
The antimicrobial potential of in vitro (callus) and in vivo (leaf and rhizome) plant parts of Himalayan peaony (Paeonia emodi) has been investigated with particular reference to solvent polarity and altitudinal variation (1827–2350 m above sea level). Five different solvents, i.e., methanol, ethanol, acetone, ethyl acetate, and hexane, and three groups of microorganisms (bacteria, actinobacteria, and fungi) were considered to detect antimicrobial potential. The qualitative estimations were done using the agar well diffusion method, while quantitative analysis was based on the dilution method. The plant parts showed significant activities against all three groups of microorganisms in qualitative bioassays. For example, ethanolic extract of leaf collected from Triyuginarayan showed the highest antibacterial activity (19.27 ± 0.23 mm) with Serratia marcescens. Maximum inhibition (13.28 ± 0.12 mm) of actinobacteria was observed in the methanolic extracts of leaf collected from Gwaldum. Methanolic extract of leaf collected from Pootiwasa showed higher antifungal activity (21.10 ± 0.06 mm). In vitro callus extract also showed activity against bacteria, actinobacteria, and Fusarrium sp. Minimum inhibitory concentrations ranged from 100 to 900 µg/mL. Pearson correlation analysis revealed that solvent polarity exhibited a significantly positive relationship with antimicrobial activity in both plant parts (leaf and rhizome).The increasing altitude negatively affected the antimicrobial activity in P. emodi plant parts. The antimicrobial activity of leaf and rhizome of P.emodi varied with the solvent types and their growing conditions. The study will have implications for developing some antifungal and antimicrobial agents, which can be used to prepare different pharmaceuticals and health products from P. emodi.
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Acknowledgements
The authors are grateful to Director GBPNIHE for extending the facilities. The National Mission on Himalayan Studies (Ministry of Environment, Forest & Climate Change, Govt. of India, New Delhi) and Inhouse project 4 is greatly acknowledge for the financial support.
Author Information
G.B. Pant National Institute of Himalayan Environment, Almora, India
kjbiotech1@gmail.com
G.B. Pant National Institute of Himalayan Environment, Almora, India
priyankaadhikariadhikari@gmail
G.B. Pant National Institute of Himalayan Environment, Almora, India
anitapandey333@gmail.com