Antidiabetic effects of fractions of methanol extract of Solanum macrocarpon Linn. On streptozotocin – induced diabetic male Wistar rats

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Doi: 10.1007/s42535-023-00751-w
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Keywords: Diabetes mellitus, Streptozotocin, n Solanum macrocarponn , Heamatological indices


Abstract


Solanum macrocarpon Linn. (SM) is a well eaten vegetable and prominent for treatment of diseases in folk medicine. This study evaluated the antidiabetic potentials of fractions of methanol extract of Solanum macrocarpon in streptozotocin – induced diabetic male Wistar rats.

Diabetes mellitus (DM) was induced in Wistar rats by single (i.p.) dose of 50 mg/kg streptozotocin (STZ) and diabetic rats were administered the extract(s) once daily for four weeks. The effect was determined on body weight (BW), blood glucose concentration (BGC), oral glucose tolerance test (OGGT), heamatological indices and lipid profiles. Also, the pancreatic level of oxidative stress and histopathology were determined. Treatment with extracts significantly decreased the elevated BGC, and improved OGTT and BW of diabetic rats. Heamatological indices, lipid profiles and pancreatic level of oxidative stress were augmented to near normalcy in diabetic rats administered the extract. Histopathology slides showed improvement in structural integrity of diabetic rats upon treatment with the extracts. The findings of this study have clearly shown that Solanum macrocarpon possess antidiabetic ability and could be used in the management of diabetes mellitus.


Diabetes mellitus, Streptozotocin, n              Solanum macrocarponn            , Heamatological indices


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References


Abdulwahab DA et al (2021) Melatonin protects the heart and pancreas by improving glucose homeostasis, oxidative stress, inflammation and apoptosis in T2DM-induced rats. Heliyon 7:e06474. https://doi.org/10.1016/j.heliyon.2021.e06474


Ajiboye BO et al (2021) Aqueous extract of Solanum macrocarpon Linn leaves abates hyperglycaemia and expression of glucose transporters gene in alloxan-induced diabetic rats. J Endocrinol Invest 44(2):265–276


Allain GC, Poon LS, Chan CS, Richmond W (1974) Quantitative determination of cholesterol using enzymatic colorimetric method. Clin Chem 20:470–475


Avwioro OG (2010) Histochemistry and tissue pathology, principle and techniques. Claverianum press, Nigeria


Boye A et al (2020) Glucose lowering and pancreato-protective effects of Abrus precatorius (L.) leaf extract in normoglycemic and STZ/ nicotinamide-induced diabetic rats. J Ethnopharmacol 258:112918. https://doi.org/10.1016/j.jep.2020.112918


Bradford MM (ed) (1976) A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254.


Cyriac B, Eswaran K (2016) Anti- hyperglycemic effect of aqueous extract of Kappaphycus alvarezii (Doty) Doty ex. P. Silva in alloxan-induced diabetic rats. J Appl Phycol 28:2507–2513


Đorđević M, Mihailović M, Arambašić et al (2017) Methanol extract protects red blood cells from oxidative damage in streptozotocin-induced diabetic rats. J Ethnopharmacol 202:172–183





Farombi EO et al (2000) Chemoprevention of 2-acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron-a Garcinia kola seed extract. Food Chem Toxicol 38:535–541


Green LC et al (1982) Analysis of nitrate, nitrite and [15 N] nitrate in biological fluids. Anal Biochem 126:131–138


Ibrahim HO et al (2018) Antidiabetic and haematological effects of Chrysophyllum albidum supplemented diet on streptozotocin induced diabetic rats. J Appl Life Sci Int 20(2):1–17


Idowu GP, Obuotor EM, Onajobi FD (2021) In vitro and in silico investigation of cholinesterase inhibition and anti-radical properties of Solanum macrocarpon leaf extracts: a preliminary anti-alzheimer’s study. Alzheimer’s Dement 17(Suppl 9). https://doi.org/10.1002/alz.049605


International Diabetes Federation (2021) The IDF Diabetes Atlas, 10th edn. Belgium


Jacobs NJ, Van Denmark PJ (1960) Determination of triglycerides. Arch Biochem Biophys 88:250–255


Jagannathan R, Neves JS, Dorcely B et al (2020) The oral glucose tolerance test: 100 years later. Diabetes Metab Syndr Obes 3787–3805


Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR (1974) Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3,4 bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11:151–169


Karigidi KO, Akintimehin ES, Omoboyowa DA, Adetuyi FO, Olaiya CO (2020) Effect of Curculigo pilosa supplemented diet on blood sugar, lipid metabolism, hepatic oxidative stress and carbohydrate metabolism enzymes in streptozotocin-induced diabetic rats. J Diabetes Metab Disord 19:1173–1184


Karigidi KO, Olaiya CO (2020a) Antidiabetic activity of corn steep liquor extract of Curculigo pilosa and its solvent fractions in streptozotocin-induced diabetic rats. J Tradit and Complement Med 10:555–564


Karigidi KO, Olaiya CO (2020b) Curculigo pilosa mitigates against oxidative stress and structural derangements in pancreas and kidney of streptozotocin-induced diabetic rats J Complement Integrat Med; 20190217


Komlaga G, Sam GH, Dickson RA, Mensah MLK, Fleischer TC (2014) Pharmacognostic studies and antioxidant properties of the leaves of Solanum macrocarpon. J Pharm Sci Res 6:1–4


Lenzen S (2017) Chemistry and biology of reactive species with special reference to the antioxidative defence status in pancreatic beta-cells. Biochim Biophys Acta Gen Subj 1861:1929–1942


Misra HP, Fridovich I (1972) The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175





Omoboyowa DA, Karigidi KO, Aribigbola TC (2020) Bridelia ferruginea Benth leaves attenuates diabetes nephropathy in STZ-induced rats via targeting NGAL/KIM-1/cystatin c gene. Clin Phytosci 6, 63 (2020)


Oyesola OA, Sampson II, Augustine AA, Adejoke OB, Taiwo GE (2022) Comparison of phytochemical constituents of ethanol leaf extracts of Solanum macrocarpon and Vernonia amygdalina. Asian J Trop Biotechnol 19:6–10




 


Acknowledgements



Author Information


Anjorin Oluwasegun Joseph
Nutritional and Industrial Biochemistry Research Laboratories, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria

Karigidi Kayode Olayele
Department of Chemical Sciences, Olusegun Agagu University of Science and Technology, Okitipupa, Nigeria


Agunloye Mary Olaoluwa
Department of Physiology, Kampala International University, Ishaka, Uganda


Olaiya Charles O.
Nutritional and Industrial Biochemistry Research Laboratories, Department of Biochemistry, University of Ibadan, Ibadan, Nigeria

cooolaiya@yahoo.com