Vegetos
(An International Journal of Plant Research & Biotechnology)
Alkaloid-rich fraction of Anacardium occidentale L. nutshells ameliorates UV-B induced oxidative damage in human peripheral blood mononuclear cells
*Article not assigned to an issue yet
Pol Arti Dilip, Wankhede Pallavi Jayant, Bagwe Akshay Dilip, Sharma Bharatbhushan
Research Articles | Published: 14 November, 2024
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Keywords: Cashew, Soxhlet extraction, SPF, Boot star rating, Antioxidant
Abstract
Humans suffer greatly from the ultraviolet (UV) radiation of the sun. A multitude of detrimental effects, such as sunburn, photoaging, hyperpigmentation, and skin cancer, are largely brought on by exposure to ultraviolet B (UV-B) radiation. These consequences can be avoided by using photoprotective agents. Recently, naturally derived products have become increasingly popular as skin photoprotective agents. Therefore, the objective of the current study was to evaluate in vitro UV-B protective activity of Anacardium occidentale L. nutshell fractions (AONFs) on human peripheral blood mononuclear cells (PBMCs). Four different AONFs, namely, aqueous, chloroform, organic, and ethyl acetate, each containing a unique combination of phytochemicals, were obtained by the Soxhlet extraction technique. The alkaloid-rich aqueous fraction displayed the highest Sun Protection Factor (SPF) and Boot Star rating, indicating its superior photoprotective efficacy. The IC50 value of the aqueous fraction on PBMCs was discovered to be 20.199 µg/ml, depicting its moderate cytotoxicity. The UV-B-irradiated cells pre-treated with an aqueous fraction demonstrated its optimum UV-protective activity at 1.8 µg/ml concentration by exhibiting the highest cell viability. Nevertheless, this concentration was found to be far less than its IC50 value. The specific activities of catalase, superoxide dismutase, and glutathione peroxidase were elevated in UV-B-irradiated cells, whereas the normal activity was documented in the irradiated cells, which are pre-treated with a 2 µg/ml fraction. Therefore, the results indicate that an alkaloid-rich aqueous fraction from cashew nut shells exhibits promising in vitro UV protective activity by displaying a wide therapeutic index and attenuating UV-induced oxidative damage.
References
Agarwal A, Cho CL, Sharma R (2018) Laboratory Evaluation of Reactive Oxygen Species. Elsevier eBooks (pp. 78–84)
Allocati N, Masulli M, DiIlio C, Federici L (2018) Glutathione transferases: substrates, inihibitors and pro-drugs in cancer and neurodegenerative diseases. Oncogenesis 7:8. https://doi.org/10.1038/s41389-017-0025-3
Chavda VP, Acharya D, Hala V, Daware S, Vora LK (2023) Sunscreens: a comprehensive review with the application of nanotechnology. J Drug Deliv SciTechnol 86:104720. https://doi.org/10.1016/j.jddst.2023.104720
Chen D, Ai X, Li Y, Li Y, Ao Y, Rong J, Li G (2023) Protective effects of Cu/Zn-SOD and Mn-SOD on UVC radiation-induced damage in NIH/3T3 cells and murine skin. Acta histochemica. https://doi.org/10.1016/j.acthis.2023.152030
de Diego GA, Penas-Steinhardt A, Ferro JP, Palacio MJ, Ossana NA, Eissa BL, Belforte F (2022) Impact of exposure to arsenic on the bacterial microbiota associated with river biofilms in the Pampas region. Aquatic Toxicol (Amsterdam, Netherlands) 252:106319. https://doi.org/10.1016/j.aquatox.2022.106319
de Jager TL, Cockrell AE, Du Plessis SS (2017) Ultraviolet light induced generation of reactive oxygen species. Adv Exp Med Biol 996:15–23. https://doi.org/10.1007/978-3-319-56017-5_2
Dunaway S, Odin R, Zhou L, Ji L, Zhang Y, Kadekaro AL (2018) Natural antioxidants: multiple mechanisms to protect skin from solar radiation. Front Pharmacol 9:392. https://doi.org/10.3389/fphar.2018.00392
Garcia-Gil M, Turri B, Gabriele M, Pucci L, Agnarelli A, Lai M, Freer G, Pistello M, Vignali R, Batistoni R, Marracci S (2021) Protopine/gemcitabine combination induces cytotoxic or cytoprotective effects in cell type-specific and dose-dependent manner on human cancer and normal cells. Pharmaceuticals (Basel) 14(2):90. https://doi.org/10.3390/ph14020090
Gęgotek A, Rybałtowska-Kawałko P, Skrzydlewska E (2017) Rutin as a mediator of lipid metabolism and cellular signaling pathways interactions in fibroblasts altered by UVA and UVB radiation. Oxid Med Cell Longev 11:1–20. https://doi.org/10.1155/2017/4721352
Geoffrey K, Mwangi AN, Maru SM (2019) Sunscreen products: rationale for use, formulation development and regulatory considerations. Saudi Pharm J 27(7):1009–1018. https://doi.org/10.1016/j.jsps.2019.08.003
Gupta A, Kumar S, Mahindroo N, Saini R (2016) Bioactive fraction from Datura stramonium Linn. promotes human immune cells mediated cytotoxicity towards lung and breast cancer cells. Pharmacogn J 8:435–439. https://doi.org/10.5530/pj.2016.5.4
Hussein E, Alhotan RA, Ebrahim A, Selim S (2023) Unraveling the potential of orange pulp for improving laying rate, egg quality, oxidative stability, fatty acids composition, and reproductive tract morphology of laying hens. Animals (Basel) 13(13):2199. https://doi.org/10.3390/ani13132199
Kaur K, Rath G (2019) Formulation and evaluation of UV protective synbiotic skin care topical formulation. J Cosmet Laser Ther 21:1–11. https://doi.org/10.1080/14764172.2019.1658878
Kolarova J, Stara A, Zuskova E, Velisek J (2022) Safety of the anthelminthic drugs levamisole, fenbendazole, and ivermectin administered in therapeutic baths for the common carp Cyprinus carpio. Vet Med (Praha) 67(7):371–378. https://doi.org/10.17221/146/2021-VETMED
Krkobabić A, Radetić M, Zille A, Ribeiro AI, Tadić V, Ilic-Tomic T, Marković D (2024) Plant-assisted synthesis of ag-based nanoparticles on cotton: antimicrobial and cytotoxicity studies. Molecules 29(7):1447. https://doi.org/10.3390/molecules29071447
Leite SB, Roosens T, El Taghdouini A, Mannaerts I, Smout AJ, Najimi M, Sokal E, Noor F, Chesne C, van Grunsven LA (2016) Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro. Biomaterials 78:1–10. https://doi.org/10.1016/j.biomaterials.2015.11.026
Li J, Hong C, Zhang H, Zhang J, Zhai R, Fei B, Zhou C (2024) Sustainable, transparent, strong toughness, UV resistance, and anti-corrosion properties of cashew shell oil-based waterborne polyurethane network derived from phloretin and sorbitan monooleate-based siloxane. Chem Eng J. https://doi.org/10.1016/j.cej.2024.151506
Mansuri R, Diwan A, Kumar H, Dangwal K, Yadav D (2021) Potential of natural compounds as sunscreen agents. Pharmacogn Rev 15:47–56. https://doi.org/10.5530/phrev.2021.15.5
Michalak M (2022) Plant-derived antioxidants: significance in skin health and the ageing process. Int J Mol Sci 23(2):585. https://doi.org/10.3390/ijms23020585
Mishra A, Kumar V, Singh A (2022) Deciphering the anti-filarial potential of bioactive compounds from Ocimum sanctum: a combined experimental and computational study. Pharm Biol 60(1):2237–2252. https://doi.org/10.1080/13880209.2022.2132030
Mobisson SK, Iyanyi UL, Ehigiator BE, Ibe FU, Monye JB, Obembe AO (2024) Elevated levels of gonadotrophic hormones and antioxidant biomarker in male rats following administration of hydromethanol leaf extract of Justicia secunda in response to 2,4-dinitrophenylhydrazine induction. J Hum Reprod Sci 17(2):112–120. https://doi.org/10.4103/jhrs.jhrs_13_24
Morocho-Jácome AL, Freire TB, de Oliveira AC, de Almeida TS, Rosado C, Velasco MVR, Baby AR (2021) In vivo SPF from multifunctional sunscreen systems developed with natural compounds-a review. J Cosmet Dermatol 20(3):729–737. https://doi.org/10.1111/jocd.13609
Mubofu EB, Mgaya JE (2018) Chemical valorization of cashew nut shell waste. Top Curr Chem (Cham) 376:8. https://doi.org/10.1007/s41061-017-0177-9
Naikodi MAR (2023) Medicinal and Aromatic Plants of India Used in the Treatment of Skin Disorders. In: Máthé Á, Khan IA (eds) Medicinal and Aromatic Plants of India, vol 2. Medicinal and Aromatic Plants of the World, Springer, Cham
Paiva-Santos AC, Gonçalves T, Peixoto D, Pires PC, Velsankar K, Jha NK, Chavda VP, Mohammad IS, Cefali LC, Mazzola PG, Mascarenhas-Melo F, Veiga F (2023) Rosacea topical treatment and care: from traditional to new drug delivery systems. Mol Pharm 20(8):3804–3828. https://doi.org/10.1021/acs.molpharmaceut.3c00324
Patias NS, de Queiroz EAIF, Ferrarini SR, Bomfim GF, Aguiar DH, Sinhorin AP, Bello AA, da Silva GVF, Cavalheiro L, Sinhorin VDG (2024) Effect of liposomal protium heptaphyllum (alb) march extract in the treatment of obesity induced by high-calorie diet. Biology (Basel) 13(7):535. https://doi.org/10.3390/biology13070535
Rocca C, De Francesco EM, Pasqua T, Granieri MC, De Bartolo A, Gallo Cantafio ME, Muoio MG, Gentile M, Neri A, Angelone T, Viglietto G, Amodio N (2022) Mitochondrial determinants of anti-cancer drug-induced cardiotoxicity. Biomedicines 10(3):520. https://doi.org/10.3390/biomedicines10030520
Sakagami H, Watanabe T, Hoshino T, Suda N, Mori K, Yasui T, Yamauchi N, Kashiwagi H, Gomi T, Oizumi T, Nagai J, Uesawa Y, Takao K, Sugita Y (2018) Recent progress of basic studies of natural products and their dental application. Medicines (Basel) 6(1):4. https://doi.org/10.3390/medicines6010004
Salehi B, Gültekin-Özgüven M, Kırkın C, Özçelik B, Morais-Braga MFB, Carneiro JNP, Bezerra CF, Silva TGD, Coutinho HDM, Amina B, Armstrong L, Selamoglu Z, Sevindik M, Yousaf Z, Sharifi-Rad J, Muddathir AM, Devkota HP, Martorell M, Jugran AK, Martins N, Cho WC (2019) Anacardium plants: chemical nutritional composition and biotechnological applications. Biomolecules 9(9):465. https://doi.org/10.3390/biom9090465
Sander M, Sander M, Burbidge T, Beecker J (2020) The efficacy and safety of sunscreen use for the prevention of skin cancer. CMAJ. https://doi.org/10.1503/2Fcmaj.201085
Santhanam RK, Ahmad S, Abas F, Safinar Ismail I, Rukayadi Y, Tayyab Akhtar M, Shaari K (2016) Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16–F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines. Molecules 21(6):652. https://doi.org/10.3390/molecules21060652
Serim I, Demirel HH, Zemheri-Navruz F, Ince S (2024) Taurine exhibits antioxidant, anti-inflammatory, and antiapoptotic effects against pyraclostrobin exposure in rats. Toxicol Res (Camb). https://doi.org/10.1093/toxres/tfae120
Shailajan S, Gurjar D (2016) Wound healing activity of Chrysophyllum cainito L. leaves: evaluation in rats using excision wound model. J Young Pharm 8:96–103. https://doi.org/10.5530/jyp.2016.2.7
Sharma B, Chauhan I (2022) In vitro and in vivo evaluation of sunscreen formulations containing nanolipid carriers. Curr Cosmetic Sci. https://doi.org/10.2174/2666779701666220819144607
Shubyr N (2023) Phytochemicals properties of herbal extracts for ultraviolet protection and skin health: a narrative review. J Radiation Res Appl Sci. https://doi.org/10.1016/j.jrras.2023.100729
Sondenheimer K, Krutmann J (2018) Novel means for photoprotection. Front Med 5:162. https://doi.org/10.3389/fmed.2018.00162
Sruthi P, Naidu MM (2023) Cashew nut (Anacardium occidentale L.) testa as a potential source of bioactive compounds: a review on its functional properties and valorization. Food Chem Adv 3:100390. https://doi.org/10.1016/j.focha.2023.100390
Tang X, Yang T, Yu D, Xiong H, Zhang S (2024) Current insights and future perspectives of ultraviolet radiation (UV) exposure: Friends and foes to the skin and beyond the skin. Environ Int 185:108535. https://doi.org/10.1016/j.envint.2024.108535
Tanveer MA, Rashid H, Tasduq SA (2023) Molecular basis of skin photoaging and therapeutic interventions by plant-derived natural product ingredients: a comprehensive review. Heliyon 9(3):e13580. https://doi.org/10.1016/j.heliyon.2023.e13580
Tavleeva MM, Belykh ES, Rybak AV, Rasova EE, Chernykh AA, Ismailov ZB, Velegzhaninov IO (2022) Effects of antioxidant gene overexpression on stress resistance and malignization in vitro and in vivo: a review. Antioxidants (Basel) 11(12):2316. https://doi.org/10.3390/antiox11122316
Telange DR, Jain SP, Pethe AM, Kharkar PS, Rarokar NR (2021) Use of combined nanocarrier system based on chitosan nanoparticles and phospholipids complex for improved delivery of ferulic acid. Int J Biol Macromol 171:288–307. https://doi.org/10.1016/j.ijbiomac.2020.12.211
Viswanath V, Leo VV, Prabha SS, Prabhakumari C, Potty VP, Jisha MS (2016) Thermal properties of tannin extracted from Anacardium occidentale L. using TGA and FT-IR spectroscopy. Nat Prod Res 30:223–227. https://doi.org/10.1080/14786419.2015.1040992
Wang P-W, Hung Y-C, Lin T-Y, Fang J-Y, Yang P-M, Chen M-H, Pan T-L (2019) Comparison of the biological impact of UVA and UVB upon the skin with functional proteomics and immunohistochemistry. Antioxidants 8(12):569. https://doi.org/10.3390/antiox8120569
Author Information
Department of Zoology, KET’s V. G. Vaze College (Autonomous), Mumbai, India