Vegetos
(An International Journal of Plant Research & Biotechnology)
Activated charcoal: an effective enhancer of in vitro seed germination and growth in Nothapodytes foetida—a threatened medicinal plant
Research Articles | Published: 25 October, 2022
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
First Page: 1049
Last Page: 1057
Views: 1438
Keywords:
Activated charcoal, Adsorption, Germination, Inhibitory, Propagation
Abstract
Nothapodytes foetida, (N. foetida) an endangered medicinal plant is a good source of camptothecin, an anti-cancer compound. Due to its indiscriminate utilization for the extraction of camptothecin, it is getting endangered. Plant tissue culture techniques offer an alternative and efficient way of propagation. In this study, we described an efficient way of in vitro seed and embryo culture of N. foetida. Murashige and Skoog’s medium supplemented with 4.4 μ mL−1 6-Benzylaminopurine (BAP) and 0.87 μ mL−1 Gibberellic acid (GA3) along with activated charcoal (0.1% w/v) was proved to be most appropriate for seed and embryo cultures. Seed germination in N. foetida was remarkably increased in the presence of activated charcoal with optimal shoot and root lengths of 14.8 cm and 11.2 cm respectively. The embryos developed into plantlets with a shoot length of 6.4 cm and root length of 3.6 cm. 100% of the seeds and embryos conversion into plantlets was achieved. The results showed that the activated charcoal is effective in absorbing inhibitory compounds in the medium and can be successfully employed for in vitro propagation of N. foetida (Wight) sleumer. The in vitro grown plants were successfully acclimatized with 87% survival rate. The in vitro protocol presented in the study can be successfully employed in the propagation of N. foetida.
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Acknowledgements
The authors wish to thank Department of Science and technology (DST)-Science and Engineering Research Board (SERB) for its financial support.
Author Information
Department of Biotechnology, National Institute of Technology, Warangal, India
srividya_jrf@nitw.ac.in
Department of Biotechnology, National Institute of Technology, Warangal, India
rathnaprabhad@nitw.ac.in