Rhizosphere metagenomics of wild and cultivated Caesalpinia bonducella L. depict similarities in their microflora

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Doi: 10.1007/s42535-022-00447-7
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Keywords: n Caesalpinia bonducellan , Microbial diversity, Oxford nanopore technology, Poly cystic ovary syndrome, Rhizosphere–rhizomicrobiome


Abstract


Exploring the rhizomicrobiome and its functional traits associated with the plant assists in enhancing the plant’s metabolites for its application in medicine. This study identified the rhizomicrobiome of wild and cultivated Caesalpinia bonducella, a potential medicinal plant used for the treatment of polycystic ovary syndrome. The microflora between the plants was assumed to vary because of the varied endurances in different culture environments. For this, soil microbial DNA was extracted from the rhizosphere regions and the 16S rDNA was sequenced using oxford nanopore technology. The results showed that the rhizomicrobiome did not vary significantly between wild and cultivated plant. The alpha diversity indices represented highly diverse bacterial communities primarily belonging to the phyla Proteobacteria, Actinobacteria, Acidobacteria and Firmicutes representing 45,018 and 48,949 operational taxonomic units in the wild and cultivated plant respectively. Furthermore, the bacterial communities categorized based on its functions could help in domestication of this plant with enhanced metabolites.


n              Caesalpinia bonducellan            , Microbial diversity, Oxford nanopore technology, Poly cystic ovary syndrome, Rhizosphere–rhizomicrobiome


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Acknowledgements


The authors wish to extend special thanks to the Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India, for providing research facility and academic assistance.


Author Information


Sivasankari Ramadurai
Department of Genetic Engineering, SRM Institute of Science and Technology, Chennai, India

Usha Balasundaram
Department of Genetic Engineering, SRM Institute of Science and Technology, Chennai, India
sundaram.usha@gmail.com