Induction of chromosomal and morphological amelioration in lentil (Lens culinaris Medik.) mutagenized population developed through chemical mutagenesis

,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00319-6
First Page: 474
Last Page: 484
Views: 367


Keywords: n Lens culinarisn , Chemical mutagenesis, Caffeine, Ethyl methane sulphonate, Cytological aberration


Abstract


Lentil (Lens culinaris Medik.) is an edible pulse crop with an excellent proportion of high-quality protein. The production of lentil should increase worldwide because of its increasing demand and significant economic value. In this present investigation, caffeine and Ethyl methane sulphonate (EMS) were used to induce morphological and cytological variations in M1 generation of lentil. The seeds of lentil L-4076 were treated to five different concentrations (0.10, 0.25, 0.50, 0.75 and 1.0%) of caffeine and EMS to develop viable variants. Various biological parameters (germination, plant survival and pollen fertility), quantitative traits, and cytological studies were performed. These parameters get affected with increasing concentration of mutagens, and sensitivity was found higher in EMS than caffeine. Cytological studies exhibited different types of meiotic abnormalities such as stickiness, laggard, bridge, desynchronisation, disturb polarity, and micronuclei. The frequency of chromosomal aberrations was much higher in the treatment of EMS as compared to caffeine.

n              Lens culinarisn            , Chemical mutagenesis, Caffeine, Ethyl methane sulphonate, Cytological aberration


*Pdf Download Buy Printed Copy

(*Only SPR Members can download pdf file; #Open Access;)

References


Alabboud I, Szilagyi L, Roman GV (2009) Molecular research on the genetic diversity of lentil genotypes (Lens culinaris Medik) using the RAPD method. Lucrări Științifice, Universitatea de Stiinte Agricole Și Medicină Veterinară" Ion Ionssescu de la Brad" Iași, Seria Agronomie 52(1): 753–757


Ali JF, Shaikh NA (2007) Genetic exploitation of lentil through induced mutations. Pak J Bot 39(7):2379–2388


Alka AMYK, Bhat TM, Choudhary S, Aslam R (2012) Genotoxic effect of ethylmethane sulphonate and sodium azide in Linum Usitatissimum L. Int J Plant Anim Environ Sci 1:1–6


Ambavane AR, Sawardekar SV, Sawantdesai SA, Gokhale NB (2015) Studies on mutagenic effectiveness and efficiency of gamma rays and its effect on quantitative traits in finger millet (Eleusine coracana L. Gaertn). J Radiat Res Appl Sci 8(1):120–125


Arulbalachandran D, Mullainathan L (2009) Chlorophyll and morphological mutants of black gram (Vigna mungo (L.) Hepper) derived by gamma rays and EMS. J Phytol 1(4):236–241


Aslam R, Bhat TM, Choudhary S, Ansari MYK, Shahwar D (2017) Estimation of genetic variability, mutagenic effectiveness and efficiency in M2 flower mutant lines of Capsicum annuum L. treated with caffeine and their analysis through RAPD markers. J King Saud Univ Sci 29(3):274–283


Auti SG (2005) Mutational Studies in mung (Vigna radiata (L.) Wilczek). Ph.D. Thesis. University of Pune, Pune (MS), India.


Avijeet C, Shukla S, Rastogi A, Mishra BK, Ohri D, Singh SP (2011) Impact of mutagenesis on cytological behavior in relation to specific alkaloids in Opium Poppy (Papaver somniferum L.). Caryologia 64(1):14–24


Benjavad Talebi A, Benjavad Talebi A (2012) Radiosensitivity study for identifying the lethal dose in MR219 (Oryza sativa L. spp. Indica cv MR219). Int J Agric Sci Res Technol Extens Edu Syst 2(2):63–68


Bhat TM, Ansari MYK, Aslam R (2012) Sodium azide (NaN3) induced genetic variation of Psoralea corylifolia L. and analysis of variants using RAPD markers. The Nucleus 55(3):149–154


Charumathi M, Rao MVB, Ravindra B, Krishna M (1992) Efficiency of early generation selection for induced micro mutations in blackgram (Vigna mungo L. Hepper). J Nucl Agric Biol 21(4):299–302


Choudhary S, Ansari MYK, Khan Z, Gupta H (2012) Cytotoxic action of lead nitrate on cytomorphology of Trigonella foenum-graecum L. Turk J Biol 36(3):267–273


Dhanavel D, Pavadai P, Mullainathan L, Mohana D, Raju G, Girija M, Thilagavathi C (2008) Effectiveness and efficiency of chemical mutagens in cowpea (Vigna unguiculata (L.) Walp). Afr J Biotechnol 7(22):4116–4117


Dixit V, Prabha R, Chaudhary BR (2013) Effects of EMS and SA on meiotic cells and thymoquinone content of Nigella sativa L. cultivars. Caryologia 66(2):178–185


Domon M, Barton B, Porte A, Rauth AM (1970) The interaction of caffeine with ultra-violet-light-irradiated DNA. Int J Radiat Biol Relat Stud Phys Chem Med 17(4):395–399


Goyal S, Khan S (2010) Induced mutagenesis in urdbean (Vigna mungo L. Hepper): a review. Int J Bot 6(3):194–206


Kavithamani D, Kalamani A, Vanniarajan C, Uma D (2008) Mutagenic effectiveness and efficiency of gamma rays and EMS in Soybean (Glycine max (L.) Merrill). Madras Agric J 95(7–12):448–451


Khan MR, Qureshi AS, Hussain SA, Ibrahim M (2005) Genetic variability induced by gamma irradiation and its modulation with gibberellic acid in M2 generation of chickpea (Cicer arietinum L.). Pak J Bot 37(2):285


Khan Z, Gupta H, Ansari MYK, Chaudhary S (2009) Methyl methanesulphonate induced chromosomal variations in a medicinal plant Cichorium intybus L. during microsporogenesis. Biol Med 1(2):66–69


Khan Z, Shahwar D, Ansari MKY, Chandel R (2019) Toxicity assessment of anatase (TiO2) nanoparticles: a pilot study on stress response alterations and DNA damage studies in Lens culinaris Medik. Heliyon 5(7):e02069


Khursheed S, Khan S (2014) Mutagenic effects of methyl methanesulphonate on the growth and yield characteristics in Lentil (Lens culinaris Medik.) var. DPL-15. Scholars Acad J Biosci 2:943–947


Khursheed T, Ansari MYK, Shahab D (2009) Studies on the effect of caffeine on growth and yield parameters in Helianthus annuus L. variety Modern. Biol Med 1(2):56–60


Kumar G, Rai P (2007) Comparative genotoxic potential of mercury and cadmium in soybean. Turk J Biol 31(1):13–18


Kumar G, Tripathi R (2008) Lead-induced cytotoxicity and mutagenicity in grass pea. Turk J Biol 32(2):73–78


Mathusamy A, Jayabalan N (2002) Effect of mutagens on pollen fertility of cotton (Gossypium hirsutum L.). Indian J Genet 62(2):187


Meshram MP, Ali RI, Patil AN, Meena S (2013) Variability studies in M3 generation in blackgram (Vigna mungo (L.) Hepper). The Bioscan 8(4):1357–1361


Minija J, Tajo A, Thoppil JE (1999) Mitoclastic properties of Mentharotun difolia L. JCytol Genet 34:169–171


Natarajan AT, Shivasankar G (1965) Studies on modification of mutation response of barley seeds to ethyl methanesulfonate. Zeitschrift für Vererbungslehre 96(1):13–21


Panigrahi KK, Mohanty A, Jyotshnarani BB (2015) Mutagenic efficiency and effectiveness of gamma rays, ethyl methane sulphonate (ems), nitrosoguanidine (ng) and their synergistic effect for different polygenic traits in black gram (Vigna mungo (l.) hepper) through induced mutagenesis. Int J Plant Anim Environ Sci 5(1):292–299


Potdukhe NR, Narkhede MN (2002) Induced mutations in pigeon pea (Cajanus cajan (L.) Millsp). J Nucl Agric Biol 31(1):41–46


Sanjai Gandhi E, Sri Devi A, Mullainathan L (2014) The effect of ethyl methane sulphonate and diethyl sulphate on chilli (Capsicum annuum L.) in M1 generation. Int Lett Natl Sci 5:18–23


Sato M, Gaul H (1967) Effect of ethyl methanesulfonate on the fertility of barley. Radiat Bot 7(1):7–15


Shah TM, Mirza JI, Haq MA, Atta BM (2006) Induced genetic variability in chickpea (Cicer arietinum L.). I. Frequency and spectrum of chlorophyll mutations. Pak J Bot 38(4):1217


Shah TM, Mirza JI, Haq MA, Atta BM (2008) Radio sensitivity of various chickpea genotypes in M1 generation I-Laboratory studies. Pak J Bot 40(2):649–665


Shahwar D, Ansari MYK, Bhat TM (2016) Assessment of genetic variability, morphology and productivity response of Vicia faba under the stress of lead nitrate. Int J Adv Life Sci 9:58–65


Shahwar D, Ansari MYK, Choudhary S, Aslam R (2017) Evaluation of yield attributing variants developed through ethyl methane sulphonate in an important proteinaceous crop-Vicia faba. Asian J. Crop Sci 9:20–27


Shahwar D, Ansari MYK, Bhat TM, Choudhary S, Aslam R (2017) Evaluation of high yielding mutant of lentil developed through caffeine of an exotic germplasm. Int J Plant Breed Genet 11:55–62


Shahwar D, Ansari MYK, Choudhary S (2018) Evaluation of genotoxic potential of heavy metal in a proteinaceous crop (Lens culinaris Medik) in aspect to cyto-morphological parameters. J Biol Sci 18:208–215


Shahwar D, Ansari MYK, Choudhary S (2019) Induction of phenotypic diversity in mutagenized population of lentil (Lens culinaris Medik) by using heavy metal. Heliyon 5(5):e01722


Shahwar D, Khan Z, Ansari MYK (2020) Evaluation of mutagenized lentil populations by caffeine and EMS for exploration of agronomic traits and mutant phenotyping. Ecol Genet Genom 14:100049


Sharma M, Khan AH, Bhat TA (2009) Assessment of mutagenicity of individual and combination treatments of gamma rays and MMS in broad bean (Vicia faba L.). Cytologia 74(2):235–241


Sinha A, Lal JP (2007) Effect of mutagens on M1 parameters and qualitative changes induced in M2 generation in lentil. Legume Res Int J 30(3):180–185


Talukdar D, Biswas AK (2007) Seven different primary trisomics in grass pea (Lathyrus sativus L.). I. Cytogenetic characterization. Cytologia 72:385–396


Waghmare VN, Mehra RB (2000) Induced genetic variability for quantitative characters in grasspea (Lathyrus sativus L.). Indian J. Genet 60(1):81–87


Wani AA, Anis M (2008) Gamma ray-and EMS-induced bold-seeded high-yielding mutants in chickpea (Cicer arietinum L.). Turk J Biol 32(3):161–166

 


Acknowledgements


The authors are thankful to chairman, Department of Botany, Aligarh Muslim University, Aligarh, India for providing facilities for carrying out this study.


Author Information


Sharma Nidhi
Department of Botany, Cytogenetics and Molecular Biology Section, Aligarh Muslim University, Aligarh, India

Choudhary Sana
Department of Botany, Cytogenetics and Molecular Biology Section, Aligarh Muslim University, Aligarh, India