Kunui Kikku, Minj Robin Anigo, Mishra Arun Kumar, Singh Satya Shila
Keywords:
Anabaena sphaerica
, Iron, Fatty acid, Lipid profile, Biodiesel agent
The ever-increasing human population of the world and its increased dependency on fossil fuels have not only decreased the limited storage of fossil fuels but also prompted the search for alternative renewable fuel sources in order to fulfil its global demand. Cyanobacteria, due to producing significant amount of lipids, have gained the attention of scientists as promising sources of biofuels. But studies related to optimization of conditions including nutrient availability, for enhancing biofuel property of cyanobacteria are still vacant. Thus, the present study was conducted to evaluate whether the differential availability of a crucial nutrient i.e. iron, exerts any impact on the biofuel properties of cyanobacteria or not. For this, a diazotrophic cyanobacterium Anabaena sphaerica was subjected to different concentrations of iron (–FeCl3, 20, 75, 100 µM FeCl3) and iron induced modulations in fatty acid composition of this organism were thoroughly studied. The results showed that the percentages of fatty acids, fatty alcohols and hydrocarbons varied at –FeCl3, 20, 75, 100 µM FeCl3. Some fatty acids (pentadecanoic acid and docosapentanoic acid) and fatty alcohols (Stearyl alcohol, 2-isopropyl-5-methyl-1-heptanol and elaidolinolenyl alcohol) have been investigated as per the available literatures. A computational analysis (PROMETHEE-GAIA) suggested that the iron deficient condition maximally increased the biofuel property of the test cyanobacterium. Results also suggested that the proficiency of A. sphaerica as a potent biodiesel agent and the synthesis of some commercially and industrially important fatty acids and its derivatives may be biotechnologically and strategically enhanced for future nutritional supplements and biofuels.
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Ambavade SD, Misar AV, Ambavade PD (2014) Pharmacological, nutritional, and analytical aspects of β-sitosterol: a review. Orient Pharm Exp Med 14:193–211
Anahas AMP, Muralitharan G (2015) Isolation and screening of heterocystous cyanobacterial strains for biodiesel production by evaluating the fuel properties from fatty acid methyl ester (FAME) profiles. Bioresour Technol 184:9–17
Cepoi L, Zinicovscaia I, Rudi L, Chiriac T, Miscu V, Djur S, Strelkova L, Vergel K, Nekhoroshkov P (2020) Growth and heavy metals accumulation by Spirulina platensis biomass from multicomponent copper containing synthetic effluents during repeated cultivation cycles. Ecol Eng 142:105637
Costa OYA, Raaijmakers JM, Kuramae EE (2018) Microbial extracellular polymeric substances: ecological function and impact on soil aggregation. Front Microbiol. https://doi.org/10.3389/fmicb.2018.01636
Dixit R, Singh S, Singh A (2020) Effect of nitrogen deficiency on the physiology and biochemical composition of microalga Scenedesmus rotundus MG910488. J Basic Microbiol 60:158–172
Fagundes MB, Falk RB, Facchi MMX, Vendruscolo RG, Maroneze MM, Zepka LQ, Jacob-Lopes E, Wagner R (2019) Insights in cyanobacteria lipidomics: a sterols characterization from Phormidium autumnale biomass in heterotrophic cultivation. Food Res Int 119:777–784
Fatma Z, Hartman H, Poolman MG, Fell DA, Srivastava S, Shakeel T et al (2018) Model-assisted metabolic engineering of Escherichia coli for long chain alkane and alcohol production. Metab Eng 46:1–12
Fillet S, Adrio JL (2016) Microbial production of fatty alcohols. World J Microbiol Biotechnol 32:152–162
Frigolet ME, Gutiérrez-Aguilar R (2017) The role of the novel lipokine palmitoleic acid in health and disease. Adv Nutr 8:173S-S181
Gaetan D, Vincent R, Philippe L (2019) The n-3 docosapentaenoic acid (DPA): a new player in the n-3 long chain polyunsaturated fatty acid family. Biochimie 159:36–48
Hanna VS, Hafez EAA (2018) Synopsis of arachidonic acid metabolism: a review. J Advanc Res 11:23–32
Hena S, Fatimah S, Tabassum S (2015) Cultivation of algae consortium in a dairy farm wastewater for biodiesel production. Water Res Ind 10:1–14
Hiltunen M, Honkanen M, Taipale S, Strandberg U, Kankaala P (2017) Trophic upgrading via the microbial food web may link terrestrial dissolved organic matter to Daphnia. J Plankt Res 39:861–869
Hoang AT (2018) Prediction of the density and viscosity of biodiesel and the influence of biodiesel properties on a diesel engine fuel supply system. J Mar Eng Technol 1–13
João AF, Squissato AL, Ribeiro MMAC, Richter EM, Faria AM, Muñoz RAA (2020) Potential of Mafura seed oil as a feedstock for biodiesel production. Biofuels 1–7
Kang JX, Wang J (2005) A simplified method for analysis of polyunsaturated fatty acids. BMC Biochem 6:1–5
Keera ST, El- Sabagh SM, Taman AR (2018) Castor oil biodiesel production and optimization. Egy J Pet 27:979–984
Khot M, Ghosh D (2017) Lipids of Rhodotorula mucilaginosa IIPL32 with biodiesel potential: oil yield, fatty acid profile, fuel properties. J Basic Microbiol 57:345–352
Kitchener RL, Grunden AM (2018) Methods for enhancing cyanobacterial stress tolerance to enable improved production of biofuels and industrially relevant chemicals. Appl Microbiol Biotechnol 102:1617–1628
Kumari N, Menghani E, Mithal R (2019) Bioactive compounds characterization and antibacterial potentials of actinomycetes isolated from rhizospheric soil. J Scient Ind Res 78:793–798
Kunui KK, Singh SS (2020) Protective role of antioxidative enzymes and antioxidants against iron-induced oxidative stress in cyanobacterium Anabaena sphaerica isolated from iron rich paddy field of Chhattisgarh India. India, J Expt Biol 58:617–630
Kunui KK, Minj RA, Singh SS (2020) Iron transportation and its impact on nitrate assimilation in the cyanobacterium Anabaena sphaerica: a promising bioaccumulator. Plant Archiv 20:3701–3709
Larranga MD, Lewis RJ, Lewis RA (2016) Hawley’s condensed chemical dictionary, 16th edn. John Wiley and Sons, Inc, Hoboken, p 530
Liu Y, Chen S, Chen J, Zhou J, Wang Y et al (2016) High production of fatty alcohols in Escherichia coli with fatty acid starvation. Microb Cell Fact 15(1):129–139
Miklaszewska M, Banaś A, Królicka A (2017) Metabolic engineering of fatty alcohol production in transgenic hairy roots of Crambe abyssinica. Biotechnol Bioeng 114:1275–1282
Peramuna A, Summeres ML (2014) Composition and occurrence of lipid droplets in the cyanobacterium Nostoc punctiforme. Arc Microbiol 196(12):881–890
Perry SC (2017) Composition with pharmacological effect for vaporizing and method. U.S. Patent 9,849,108
Prasath KG, Sethupathy S, Pandian SK (2019) Proteomic analysis uncovers the modulation of ergosterol, sphingolipid and oxidative stress pathway by myristic acid impeding biofilm and virulence in Candida albicans. J Proteom 208:103503–103522
Rahman Z, Nawab J, Sung BH, Kim SC (2018) A critical analysis of bio-hydrocarbon production in bacteria: current challenges and future directions. Energies 11:2663–2677
Rajeshwari KR, Rajashekhar M (2011) Biochemical composition of seven species of cyanobacteria isolated from different aquatic habitats of Western Ghats. Southern Indian 54(5):849–857
Saraf S, Thomas B (2007) Influence of feedstock and process chemistry on biodiesel quality. Process Saf Environ 85:360–364
Shi K, Gao Z, Shi TQ, Song P, Ren LJ, Huang H et al (2017) Reactive oxygen species-mediated cellular stress response and lipid accumulation in oleaginous microorganisms: the state of the art and future perspectives. Fron Microbiol 8:793–792
Singh SS, Singh P, Kunui K, Minj RA (2014) Diversity analysis and distribution pattern of cyanobacteria isolated from different sites of Chhattisgarh, India. J Asia Pac Biodivers 7:462–470
Singh S, Verma E, Tiwari B, Niveshika MAK (2017) Modulation of fatty acids and hydrocarbons in Anabaena 7120 and its ntcA mutant under calcium. J Basic Microbiol 57:171–183
Sinha SK, Gupta A, Bharalee R (2016) Production of biodiesel from freshwater microalgae and evaluation of fuel properties based on fatty acid methyl ester profile. Biofuels 7:69–78
Thangavel K, Krishnan PR, Nagaiah S, Kuppusamy S, Chinnasamy S, Rajadorai JS et al (2018) Growth and metabolic characteristics of oleaginous microalgal isolates from Nilgiri biosphere Reserve of India. BMC Microbiol 18:1–17
Tomer AK, Dadheech PK (2020) Bioprospecting antioxidants in some non-heterocystous filamentous cyanobacteria inhabit water bodies of semi-arid Rajasthan in India. Vegetos 33(3):601–609
Verma E, Chakraborty S, Singh SS, Mishra AK (2019) Salinity-induced oxidative stress-mediated change in fatty acids composition of cyanobacterium Synechococcus sp. PCC7942. Inter J Environ Sci Technol 16:875–886
Vernieri C, Casola S, Foiani M, Pietrantoni F, de Braud F, Longo V (2016) Targeting cancer metabolism: dietary and pharmacologic interventions. Cancer Disco 6:1315–1333
Vishwanath SJ, Kosma DK, Pulsifer IP, Scandola S, Pascal S, Joubès J, Dittrich-Domergue F, Lessire R, Rowland O, Domergue F (2013) Suberin-associated fatty alcohols in Arabidopsis: distributions in roots and contributions to seed coat barrier properties. Plant Physiol 163:1118–1132
We thank the Head, Department of Botany, Banaras Hindu University, Varanasi and Guru Ghasidas Vishwavidyalaya, Bilaspur for providing the necessary facilities and encouragement. The authors are also grateful to Institute of Eminence (IoE-6031), BHU for financial assistance.