Improved micropropagation, morphometric traits and photosynthetic pigments content using liquid culture system in Spathoglottis plicata Blume

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-021-00303-0
First Page: 9
Last Page: 18
Views: 534

Keywords: Acclimatization, Chlorophylls, Garden orchid, In vitro, Protocorms


An effective plant regeneration protocol via a liquid culture system has been ascertained in Spathoglottis plicata Blume (Orchidaceae), an important orchid of the horticulture industry. The seeds were germinated asymbiotically on the agar-gelled Murashige and Skoog’s (MS) medium supplemented with 1.0 mg L− 1 6-benzylaminopurine (BAP) and about 93.0 % seeds were germinated via the formation of protocorms. The highest rate of shoot proliferation (72.3 ± 0.26 shoots with average 8.0 ± 0.19 cm length per protocorm after 4th subculture) was obtained with liquid MS medium containing 1.0 mg L− 1 BAP and 0.5 mg L− 1 α-naphthalene acetic acid (NAA). The liquid culture system also enhanced the qualitative foliar morphometric parameters (7.3 ± 0.17 cm length x 1.2 ± 0.25 cm width). Half strength MS medium fortified with 1.5 mg L− 1 indole-3-butyric acid (IBA) was observed most suitable for rooting of the shoots. The shoots derived through liquid culture responded better (94.0 %) in rooting experiments (13.0 ± 0.22 roots per shoot, 4.0 ± 0.25 cm length), as opposed to 79.0 % in semi-solid medium with 6.5 ± 0.29 roots and 3.3 ± 0.19 cm length. Additionally, the liquid culture system enhanced qualitative and quantitative parameters of the shoot and root systems, and photosynthetic pigments (chlorophyll a, b, and carotenoids) which assisted in acclimatization and survival success of plantlets (100 %). This is the first attempt to use of the liquid medium for in vitro regeneration of S. plicata which could be adopted for large-scale propagation and conservation of this important but vulnerable orchid species.

Acclimatization, Chlorophylls, Garden orchid, In vitro, Protocorms

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Authors MSS and PS are grateful to the National Medicinal Plants Board, Ministry of AYUSH, Government of India for providing financial support to their laboratory (grant number NMPB/IFD/GIA/NR/PL/2018-19/187).

Author Information

Shekhawat Mahipal S.
Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, India
Priyadharshini S.
Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, India

Manokari M.
Siddha Clinical Research Unit, Central Council for Research in Siddha (M/o AYUSH), Palayamkottai, India