Establishment of adventitious root culture from leaf explants of Plumbago zeylanica: an endangered medicinal plant

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Short Communications | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-021-00300-3
First Page: 276
Last Page: 280
Views: 528

Keywords: n Plumbago zeylanican , Adventitious root, Micropropagation, Endangered, In vitro


Plumbago zeylanica L. (Plumbaginaceae) is a very important medicinal plant containing plumbagin as the major secondary metabolite synthesized and stored in its roots. Over-exploitation to meet the growing demand on global level has put it under the endangered plant category. The objective of the present study is to provide the desired plant material for industrial use. Adventitious root culture is considered as an alternative source of plant material. In the present study, the leaf explants were inoculated on half-strength Murashige and Skoog (MS) medium supplemented with different plant growth regulators to obtain the adventitious roots in vitro. The leaf explants inoculated on half-strength MS medium supplemented with 6.0 µM 6-benzylaminopurine (BAP) and 2.5 µM 1-naphthaleneacetic acid (NAA) produced maximum roots within the shortest period of time. The current study aims at the optimization of in vitro adventitious roots initiation and growth of P. zeylanica from the leaf explants. The protocol established here can be used for bulk production of P. zeylanica roots for medicinal use.

n              Plumbago zeylanican            , Adventitious root, Micropropagation, Endangered, In vitro

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Author Information

Katoch Kajal
Department of Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Phagwara, India

Nazir Romaan
Department of Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Phagwara, India

Kumar Vijay
Department of Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Phagwara, India

Sanyal Rupa
Department of Botany, Bhairab Ganguly College, Kolkata, India

Dey Abhijit
Department of Life Sciences, Presidency University, Kolkata, India
Pandey Devendra Kumar
Department of Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Phagwara, India