Comparative root proteome analysis of two contrasting wheat genotypes Kharchia-65 (highly salt-tolerant) and PBW-373 (salt-sensitive) for salinity tolerance using LC–MS/MS approach


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-021-00292-0
First Page: 133
Last Page: 139
Views: 394

Keywords: Liquid chromatography–mass spectrometry, Salinity tolerance, Proteomics, Wheat varieties KH-65 and PBW-373


Biochemical studies show a definite relation of salinity stress with plant proteome alteration. The impact of salinity stress on the root proteins is elucidated here. The experiment included two genotypes of wheat, KH-65 and PBW-373; this experiment was tested against 0 and 300 mM NaCl for 48 h. Liquid chromatography combined with mass spectrometry (LC–MS/MS) was employed to analyze extracted proteins. Significant results were obtained regarding the rarely high number of proteins, peptides, and protein groups as the chromatograms of two genotypes revealed as many as 21,863 proteins, 5133 protein groups, and 27,881 peptides. Analysis of genotype-specific variations shows that change in the expression pattern of proteins corresponds with different levels of salinity tolerance as seen through the overwhelming higher up-regulation in tolerant genotype (KH-65) and comparative down-regulation of proteins in susceptible genotype (PBW-373). Some of such proteins, auxin-responsive protein, peroxidase and vacuolar protein sorting-associated protein 41 homolog, are mentioned.

Liquid chromatography–mass spectrometry, Salinity tolerance, Proteomics, Wheat varieties KH-65 and PBW-373

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The authors highly acknowledge the Indian Institute of Wheat and Barley Research (IIWBR), Karnal and Central Soil Salinity Research Institute (CSSRI), Karnal, Haryana, India, for providing wheat germplasm for the present study. In addition, the financial assistance to RY in the form of a University Research Scholarship by Maharshi Dayanand University (MDU) is also gratefully acknowledged.

Author Information

Yadav Renu
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, India

Singh Nater Pal
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, India