Optimization of production parameter and application of glycoprotein produced from Sphingobacterium thalpophilum DP9

Patel, Dhaval, Ingale, Snehal, Nataraj, M.

Research Articles | Published: 28 September, 2021

Volume: 35, Issue: 1, March 2022
Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00296-w
First Page: 26
Last Page: 37
Views: 208

Keywords: Biosurfactant, Central Composite Design, Cosmetics, Glycoprotein, Shampoo


Biosurfactants are biologically secreted amphiphilic molecules which resemble with the chemical surfactant in function. Compared to the chemical surfactants, they are biodegradable, biocompatible and less toxic. However, commercialization of biosurfactant production is still challenging due to high production and recovery cost, less efficient strain availability, and less knowledge about biosynthetic or metabolic pathways of biosurfactant production. Therefore, the attempt was made to optimize the production parameter for improving the biosurfactant production using Central Composite Design (CCD) from Sphingobacterium thalpophilum DP9. In addition, application of biosurfactant as shampoo ingredient was also studied. Results showed that the biosurfactant production was increased by 4.94 fold when 1% bacterial inoculum was inoculated in Bushnell-Hass medium (pH–11) supplemented with 8 g% sucrose. Biosurfactant was effectively purified by acid precipitation from cell free supernatant. Chromatographic techniques (TLC and HPTLC) and Native–PAGE electrophoresis confirmed glycoprotein nature of the biosurfactant with 85.75 ± 0.05% carbohydrate and 4.58 ± 0.03% protein part. In addition, two fragments of protein (37 kD and 44 kD) were also observed in electrophoretic analysis. According to the ‘blind touch test’ data of volunteer it was noted that the biosurfactant based shampoo showed satisfactory (conditioning) activity compared to commercially available shampoo. Therefore, it was concluded that glycoprotein produced from S. thalpophilum DP9 can be used in cosmetic or personal health products.

Biosurfactant, Central Composite Design, Cosmetics, Glycoprotein, Shampoo

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Authors are thankful to the Head of Department, Post Graduate Department of Biosciences and Sardar Patel University (Vallabh vidyanagar, Anand, Gujarat, India) for providing the platform to carry out this research work.

Author Information

Patel, Dhaval
Post Graduate Department of Bioscience, Sardar Patel University, Anand, India
Ingale, Snehal
Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Applied Sciences, Anand, India

snehalingale@aribas.edu.in Nataraj, M.
Post Graduate Department of Bioscience, Sardar Patel University, Anand, India