Biomass and camptothecin production in the calcium chloride elicited and liquid medium overlayed Nothapodytes nimmoniana (J. Graham) Mabberly callus cultures

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Doi: 10.1007/s42535-021-00293-z
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Last Page: 114
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Keywords: Plant secondary metabolites, Phytochemicals, Elicitation, Calcium chloride, Cell culture, Liquid overlays


Abstract


Nothapodytes nimmoniana (Icacinaceae) is an endangered medicinal plant species and a convenient source for the large-scale isolation of anti-cancer alkaloid camptothecin (CPT). Application of in vitro cultures elicitation is a promising strategy for enhancing the production of the alkaloid to obtain higher yield. In the present experiment, effects of calcium chloride elicitor on biomass and CPT production was studied at 10, 15, 20, 25, and 30 mM concentrations after 15, 30, and 45 days cultivation using solid and with a liquid medium overlay of Murashige and Skoog medium supplemented with N6-benzylaminopurine and naphthalene acetic acid (2.0 + 1.0 mg L−1). Maximum production of biomass and CPT was achieved with solid medium cultivation of the callus cultures overlayed with 1 mL liquid medium and CaCl2 elicitor at 25 mM. In these cultures, a maximum of 14.7-fold enhanced production of the alkaloid was achieved relative to the control. Higher concentrations of the CaCl2 elicitor resulted in a suppressive effect on the parameters studied relative to other treatment concentrations tested. The experimental results obtained suggest elicitation is an alternative strategy that could enhance production of CPT in the in vitro cultures of N. nimmoniana.


Plant secondary metabolites, Phytochemicals, Elicitation, Calcium chloride, Cell culture, Liquid overlays


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Acknowledgements


Authors are grateful to Jamia Hamdard New Delhi, India for support to the research work.


Author Information


Isah Tasiu
Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India
taasmore@yahoo.co.uk
Masood Samiya
Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India


Umar Shahid
Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India