Evaluation of divergence in underutilized wonder legume winged bean [Psophocarpus tetragonolobus (L.) DC.] using ISSR markers


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-021-00282-2
First Page: 140
Last Page: 148
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Keywords: Underutilized legume, Genetic variation, Molecular markers


Abstract


Winged bean (Psophocarpus tetragonolobus (L.) DC) is a nutritious tropical legume known for its high protein content in the seeds. Twenty winged bean genotypes were studied for the genetic diversity using Inter-simple sequence repeat (ISSR) markers. On ISSR analysis, 124 bands were generated by 11 informative primers, of which 76 bands were polymorphic and 3 were unique. The PIC values of ISSR primers studied ranged from 0.06 to 0.39; resolving power (Rp) values ranged from 0.9 to 4.7 and the mean number of bands per locus ranged from 9.6 to 18.2. The mean number of bands per accession ranged from 3.9 to 13.7. Narrow genetic base was revealed with the similarity values amongst genotypes ranging from 0.72 to 0.98; with 64 combinations having similarity value more than 0.90 and another 105 genotypic combinations having similarity index in between 0.80 and 0.90. The dendrogram showed two major clusters with 4 groups; with cluster-I being mono-genotypic (EC142666); while two little bit divergent groups could be clearly distinguished from highly homogenous core twelve genotypes. The principal component analysis (PCA) derived pattern was almost in accordance with the clustering pattern based dendrogram. Within the narrow base of the winged bean genotypes studied; four main groups were observed with EC142666 being most divergent; followed by two diverse groups comprising of 3 (EC-178268/EC-178269/EC-178311) and 4 (EC-121921/EC-178291/EC-178310/EC-251020) genotypes respectively; with the other genotypes were very close to each other indicating genetic bottleneck. This necessitates the need for identification of divergence germplasm for breeding to broaden the genetic base.

Underutilized legume, Genetic variation, Molecular markers


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Acknowledgements


Authors are thankful to authorities of Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, India for providing necessary facilities to undertake this work. Help provided by our colleagues during this study is also acknowledged.


Author Information


Dieu Tran Quang
Department of Agricultural Botany, Mahatma Phule Krishi Vidyapeeth, District Ahmednagar, India