Morpho-taxonomical and karyological study of Gopi banana: an indigenous palatable cultivated variety of Musa × paradisiaca

, , , , ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00267-1
First Page: 937
Last Page: 943
Views: 1433


Keywords: Musa , Morphology, Taxonomy, Cytology, Karyotype, Gopi


Abstract


Tripura has a good grasp of edible cultivated bananas which are yet to be treated on scientific grounds. Amongst them, one is locally named as ‘Gopi Kola’ which has been domesticated by the farmers due to its good vigor and yield. However, the technical identity of the Musa variety Gopi is indistinct. The present investigation addresses the identification and documentation of Musa variety Gopi in the perspectives of morphology and cytology. Systematic morphological screening and cytological analysis produced relevant results in decoding its taxonomical position. The taxonomical scorecard method reveals a score of 61 according to which the genome based grouping is ABB for the variety. Analysis of morphological traits showing concurrence with both the ancestral species also signifies its hybrid nature. Chief distinguishing features of the cultivated variety Gopi include shiny pseudostem with light blotches, light waxy nature of male bud, thick finger peel and sweet fleshy pulp with occasional presence of few soft seeds mostly at the upper portion of the finger. Cytological results implying 2n = 3x = 33 chromosome count also adds to the triploidy of the cultivar. Karyotype is symmetrical belonging to Stebbins 2A category and is characterized by prevalence of median and submedian chromosomes with three secondary constricted chromosomes. On account of revelations in the present study, the identity of Musa cultivar Gopi is designated as Musa × paradisiaca L. ‘Gopi’.



                Musa
              , Morphology, Taxonomy, Cytology, Karyotype, Gopi


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References


Atom AD, Lalrinfela P, Thangjam R (2015) Genome classification of banana genetic resources of Manipur using morphological characters. Sci Vis 15(4):189–194


Brickell CD, Alexander C, Cubey JJ, David JC, Hoffman MHA, Leslie AC, Malecot V, Xiaobai J (2016) Conventions for presentation of names. In: International code of nomenclature for cultivated plants (ICNCP or cultivated plant code). Scripta Hort number 18, 9th edn. International Society for Horticultural Society. ISBN 978-94-6261-116-0


Capdeville GD, Souza Junior MT, Szinay D, Cardamone Diniz LE, Wijnker E, Swennen R, Jan Kema GH, Jong HD (2009) The potential of high resolution BAC-FISH in banana breeding. Euphytica 166:431–443


Chester M, Leitch AR, Soltis PS, Soltis DE (2010) Review of the application of modern cytogenetic methods (FISH/GISH) to the study of reticulation (polyploidy/hybridization). Genes 1(2):166–192. https://doi.org/10.3390/genes1020166


Christelova P, Valarik M, Hribova E, Van den houwe I, Channeliere S, Roux N, Doležel J (2011) A platform for efficient genotyping in Musa using microsatellite markers. AoB Plants 2011:plr024. https://doi.org/10.1093/aobpla/plr024


Christelova P, De Langhe E, Hribova E, Cizkova J, Sardos J, Huskova M, Van den houwe I, Sutanto A, Kepler AK, Swennen R, Roux N, Dolezel J (2017) Molecular and cytological characterization of the global Musa germplasm collection provides insights into the treasure of banana diversity. Biodivers Conserv 26:801–824


Cornejo JC, Rosello S, Nuez F (2013) Phenotypic and genetic diversity of Spanish tomato landraces. Sci Hortic 162:150–164


De Melo CAF, Souza MM, Silva GS (2017) Karyotype analysis by FISH and GISH techniques on artificial backcrossed interspecific hybrids involving Passiflora sublanceolata (Killip) MacDougal & Passifloraceae). Euphytica 213:161


Ghosh S, Das A, Ghorai A, Jha TB (2013) Comparative karyomorphology of edible Musa cultivars of West Bengal. Caryologia Int J Cytol Cytosyst Cytogenet 66(3):243–250


Guillermo JS, Aveliano F (2003) Karyotype analysis and chromosome evolution in South American species of Lathyrus (Leguminosae). Am J Bot 90(7):980–987


Haider N (2011) Identification of plant species using traditional and molecular-based methods. In: Davis RE (ed) Wild plants: identification, uses and conservation. Nova Science Publishers, pp 1–62


Hapsari L, Wahyudi D, Azrianingsih R, Arumingtyas EL (2015) Genome identification of bananas (Musa L. from East Java Indonesia assessed with PCR-RFLP of the internal transcribed spacers nuclear ribosomal DNA. Int J Biosci 7(3):42–52


Heslop-Harrison JS (2011) Genomics, banana breeding and super domestication. Acta Hortic 897:55–62


Huziwara Y (1962) Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosome of Aster. Am J Bot 49:116–119


Lalrinfela PC, Thangjam R (2012) Genome characterization of banana genetic resources of Mizoram, India. Sci Vis 12(1):32–36


Levan AK, Fredga K, Sandberg A (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220


Manzo-Sánchez G, Buenrostro-Nava MT, Guzmán-González S, Orozco-Santos V, Muhammad Y, Rosa M, Escobedo-Gracia M (2015) Genetic diversity in bananas and plantains (Musa spp.). In: Caliskan M, Oz GC, Kavakli IH, Ozcan B (eds) Molecular approaches to genetic diversity. IntechOpen. https://doi.org/10.5772/59421


Nair AS, Teo CH, Schwaracher T, Harrison PH (2005) Genome classification of banana cultivars from south India using IRAP markers. Euphytica 144:285–290


Nazari Z, Mirzaien H, Bakhshi KGR, Asadicorom F (2012) Karyotypic characteristics of Moringa peregrina (forssk.) Fiori in Iran. Iran J Med Aromat Plants 27(4):635


Opara UL, Jacobson D, Al-Saady NA (2010) Analysis of genetic diversity in banana cultivars (Musa cvs.) from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses. J Zhejiang Univ Sci 11(5):332–341


Osuji JO (1998) Chromosomal investigations of infra-specific taxa in Musa paradisiaca L. (plantain; Musa spp. AAB group. J Innov Life Sci 3:87–92


Paszko B (2006) A critical review and a new proposal of karyotype asymmetry indices. Plant Syst Evol 258:39–48


Peruzzi L, Eroglu HE (2013) Karyotype asymmetry: again, how to measure and what to measure? Comp Cytogenet 7:1–9


Phanideepthi V (2016) Taxonomic scoring and genomic grouping in bananas. Flora Fauna 22(2):151–158


Sahin E, Eroglu HE, Hamzaoglu E, Koc M (2016) Karyotype analysis of four species of dianthus section Fimbriati (Caryophyllaceae, Sileneae). Caryologia 69:3.267–272


Sharma AK, Sharma A (1980) Chromosome technique theory and practical, 3rd edn. Butter works Ltd, London


Simmonds NW, Shepherd K (1955) The taxonomy and origins of the cultivated bananas. Linnean Soc Bot 55:302–312


Singh HP, Uma S (1996) Genetic diversity of banana in India. In: The proceedings of the conference on challenges for banana production and utilization in 21st century, Trichy, Sept, pp 24–25


Singh HP, Uma S, Sathiamoorthy S (2001) A tentative key for identification and classification of Indian bananas. Director, National Research Centre for Banana, Indian Council of Agricultural Research, Publ, Trichy India, pp 1–61


Singh WR, Singh SS, Shrivastava K (2014) Analysis of banana genome groups of wild and cultivated cultivars of Manipur, India using sScore card method. Adv Appl Sci Res 5(1):35–38


Singh WA, Singh NS, Handique PJ, Devi HR (2015) Morpho-taxonomical and molecular assessment of Musa genotypes from north-east India by morphological and inter-retrotransposon amplified polymorphism markers. Plant Syst Evol 301:563–575


Stebbins GL (1971) Chromosomal evolution in higher plants. Edward Arnold, London


Stover RH, Simmmonds NW (1987) Bananas, 3rd edn. Longman scientific and Technical, Essex, p 468


Sunandar A, Kahar AP (2017) Morphological and anatomical characteristics of Pisang awak (Musa paradisiaca cv. Awak) in West Kalimantan. Biosaintifika 9(3):579–584 


Uma S, Shyam B, Selvarajan R, Sathiamoory S (2001) Collection and characterization of banana and plantains of northeast India. Adv Banana Plantain R&D Asia Pac 10:103–109


Uma S, Siva SA, Saraswathi MS, Manickavasagam M, Durai P, Selvarajan R, Sathiamoorthy S (2006) Variation and interspecific relationships in Indian wild Musa balbisiana (BB) population as evidenced by random amplified polymorphic DNA. Genet Resour Crop Evol 53:349–355


Valmayor RV, Jamaluddin SH, Silayoi B, Kusumo S, Danh LD, Pascua OC, Espino RRC (2000) Banana cultivar names and synonyms in Southeast Asia. International Network for the improvement of Banana and Plantain-Asia and the pacific office INIBAP, Los banos, Laguna, Phillipines. ISBN 971-91751-2-5


Watanabe K, Yahara T, Denda T, Kosuge K (1999) Chromosomal evolution in the genus Brachyscome (Asteraceae, Astereae): statistical tests regarding correlation between changes in karyotype and habitusing phylogenetic information. J Plant Res 112:145–161


Zarco CR (1986) A New method for estimating karyotype asymmetry. Taxon 35(3):526–530

 


Acknowledgements


The authors are thankful to the Department of Biotechnology (DBT), Government of India for providing financial support through a project (No. BCIL/NER-BPMC/2018/245) for conducting this research work.


Author Information


Nath Bibhash
Cytogenetics and Plant Biotechnology Lab, Department of Botany, Tripura University, Suryamaninagar, India

Saha Puja Rani
Cytogenetics and Plant Biotechnology Lab, Department of Botany, Tripura University, Suryamaninagar, India
pujars4@yahoo.com

Das Anath Bandhu
Department of Botany, Utkal University, Bhubaneswar, India


Jena Satyanarayan
Genetics and Molecular Biology, CSIR-National Botanical Research Institute, Lucknow, India


Sinha Sangram
Cytogenetics and Plant Biotechnology Lab, Department of Botany, Tripura University, Suryamaninagar, India

Sinha Rabindra Kumar
Cytogenetics and Plant Biotechnology Lab, Department of Botany, Tripura University, Suryamaninagar, India